Localisation and distance between ABL and BCR genes in
interphase nuclei of bone marrow cells of control donors and
patients with chronic myeloid leukemia

J 1997

Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukemia

LUKÁŠOVÁ, Emilie, Stanislav KOZUBEK, Michal KOZUBEK, Jana KJERONSKÁ, Leonard RÝZNAR et. al.

Basic information

Original name

Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukemia

Authors

LUKÁŠOVÁ, Emilie (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Jana KJERONSKÁ, Leonard RÝZNAR, Jana HORÁKOVÁ, Eva KRAHULCOVÁ (203 Czech Republic) and Gerda HORNECK

Edition

Human Genetics, 1997, 0340-6717

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.662

RIV identification code

RIV/00216224:14330/97:00001513

Organization unit

Faculty of Informatics

Keywords in English

nuclear topography; ABL; BCR; interphase nuclei; bone marrow; CML

Abstract

V originále

Quantitative measurements of the nuclear localisation of the ABL and BCR genes and the distance between them were performed in randomly oriented bone marrow cells of control donors and patients with chronic myeloid leukaemia (CML). Most ABL and BCR genes (75%) are located at a distance of 20-65% of the local radius from the nuclear centre to the nuclear membrane. A chimeric BCR-ABL gene located on a derivative chromosome 22 resulting from t(9;22)(q34;q11) [the so-called Philadelphia (Ph) chromosome] as well as the intact ABL and BCR genes of patients suffering from chronic myeloid leukemia are also located mostly in this region, which has a mean thickness of 2 ľm in bone marrow cells. We have not found any significant differences in the location of the two genes in the G1 and G2 phases of the cell cycle, nor between bone marrow cells and stimulated lymphocytes. Irradiation of lymphocytes with a dose of 5 Gy of gamma-rays results in a shift of both genes to the central region of the nucleus (0-20% of the radius distant from the nuclear centre) in about 15% of the cells. The minimum distance between one ABL and one BCR gene is less than 1 ľm in 47.5% of bone marrow cells of control donors. Such a small distance is found between homologous ABL and between homologous BCR genes in only 8.1% and in 8.4% of cells, respectively. It is possible that the relative closeness of nonhomologous ABL and BCR genes in interphase nuclei of bone marrow cells could facilitate translocation between these genes. In 16.4% of bone marrow cells one ABL and one BCR gene are juxtaposed (the distance between them varies from 0-0.5 ľm) and simulate the Ph chromosome. This juxtaposition is the result of the projection of two genes located one above another into a plane, as follows from the probability calculation.

Links

GA202/96/1718, research and development project

Name: Stanovení stabilních chromosomálních aberací indukovaných v buňkách lidské krve hustě ionizujícím zářením

Investor: Czech Science Foundation, Detections of stabile chromosome aberration induced by densely ionizing radiation in human blood cells

GA202/97/0874, research and development project

Name: Struktura interfázního jádra a její změny po ozáření

Investor: Czech Science Foundation, The structure of interphase nucleus and its changes after irradiation

Citovat

LUKÁŠOVÁ, Emilie, Stanislav KOZUBEK, Michal KOZUBEK, Jana KJERONSKÁ, Leonard RÝZNAR, Jana HORÁKOVÁ, Eva KRAHULCOVÁ and Gerda HORNECK. Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukemia. Human Genetics. 1997, vol. 100, 5/6, p. 525-535. ISSN 0340-6717.

@article{224672,
   author = {Lukášová, Emilie and Kozubek, Stanislav and Kozubek, Michal and Kjeronská, Jana and Rýznar, Leonard and Horáková, Jana and Krahulcová, Eva and Horneck, Gerda},
   article_number = {5/6},
   keywords = {nuclear topography; ABL; BCR; interphase nuclei; bone marrow; CML},
   language = {eng},
   issn = {0340-6717},
   journal = {Human Genetics},
   title = {Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukemia},
   volume = {100},
   year = {1997}
}

TY  - JOUR
ID  - 224672
AU  - Lukášová, Emilie - Kozubek, Stanislav - Kozubek, Michal - Kjeronská, Jana - Rýznar, Leonard - Horáková, Jana - Krahulcová, Eva - Horneck, Gerda
PY  - 1997
TI  - Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukemia
JF  - Human Genetics
VL  - 100
IS  - 5/6
SP  - 525
EP  - 525
SN  - 03406717
KW  - nuclear topography
KW  - ABL
KW  - BCR
KW  - interphase nuclei
KW  - bone marrow
KW  - CML
N2  - Quantitative measurements of the nuclear localisation of the ABL and BCR genes and the distance between them were performed in randomly oriented bone marrow cells of control donors and patients with chronic myeloid leukaemia (CML). Most ABL and BCR genes (75%) are located at a distance of 20-65% of the local radius from the nuclear centre to the nuclear membrane. A chimeric BCR-ABL gene located on a derivative chromosome 22 resulting from t(9;22)(q34;q11) [the so-called Philadelphia (Ph) chromosome] as well as the intact ABL and BCR genes of patients suffering from chronic myeloid leukemia are also located mostly in this region, which has a mean thickness of 2 ľm in bone marrow cells. We have not found any significant differences in the location of the two genes in the G1 and G2 phases of the cell cycle, nor between bone marrow cells and stimulated lymphocytes. Irradiation of lymphocytes with a dose of 5 Gy of gamma-rays results in a shift of both genes to the central region of the nucleus (0-20% of the radius distant from the nuclear centre) in about 15% of the cells. The minimum distance between one ABL and one BCR gene is less than 1 ľm in 47.5% of bone marrow cells of control donors. Such a small distance is found between homologous ABL and between homologous BCR genes in only 8.1% and in 8.4% of cells, respectively. It is possible that the relative closeness of nonhomologous ABL and BCR genes in interphase nuclei of bone marrow cells could facilitate translocation between these genes. In 16.4% of bone marrow cells one ABL and one BCR gene are juxtaposed (the distance between them varies from 0-0.5 ľm) and simulate the Ph chromosome. This juxtaposition is the result of the projection of two genes located one above another into a plane, as follows from the probability calculation.
ER  -

LUKÁŠOVÁ, Emilie, Stanislav KOZUBEK, Michal KOZUBEK, Jana KJERONSKÁ, Leonard RÝZNAR, Jana HORÁKOVÁ, Eva KRAHULCOVÁ and Gerda HORNECK. Localisation and distance between ABL and BCR genes in interphase nuclei of bone marrow cells of control donors and patients with chronic myeloid leukemia. \textit{Human Genetics}. 1997, vol.~100, 5/6, p.~525-535. ISSN~0340-6717.

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