Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?

DOSEDĚLOVÁ, Věra, Marketa LASTOVICKOVA, Juan F AYALA-CABRERA, Jiří DOLINA, Štefan KONEČNÝ, Oliver J. SCHMITZ a Petr KUBÁŇ. Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus? Journal of Chromatography A. Amsterdam: ELSEVIER SCIENCE BV, roč. 1676, August 2022, s. 1-13. ISSN 0021-9673. doi:10.1016/j.chroma.2022.463287. 2022.

Základní údaje

• Originální název: Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?
• Autoři: DOSEDĚLOVÁ, Věra (203 Česká republika, domácí), Marketa LASTOVICKOVA (203 Česká republika), Juan F AYALA-CABRERA, Jiří DOLINA (203 Česká republika, domácí), Štefan KONEČNÝ (703 Slovensko, domácí), Oliver J. SCHMITZ a Petr KUBÁŇ (203 Česká republika).
• Vydání: Journal of Chromatography A, Amsterdam, ELSEVIER SCIENCE BV, 2022, 0021-9673.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30219 Gastroenterology and hepatology
• Stát vydavatele: Nizozemské království
• Utajení: není předmětem státního či obchodního tajemství
• WWW: URL
• Impakt faktor: Impact factor: 4.100
• Kód RIV: RIV/00216224:14740/22:00128067
• Organizační jednotka: Středoevropský technologický institut
• Doi: http://dx.doi.org/10.1016/j.chroma.2022.463287
• UT WoS: 000834927200006
• Klíčová slova anglicky: Barrett’s esophagus; Bileacids; LC-MS; Salivaanalysis; Solid-phaseextraction
• Štítky: 14110213, podil, rivok
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Bile acids are a group of steroid compounds essential for lipid digestion. However, when bile acids are refluxed into the stomach and the esophagus, during the so called duodenogastroesophageal reflux, they can have a detrimental effect on the esophageal epithelium and cause pathological changes of esophageal tissue, e.g., Barrett's esophagus (BE). The levels of bile acids in saliva could therefore serve as possi-ble biomarkers for the diagnostics of BE. In this work, we focused on optimization of sample collection and preparation by solid-phase extraction and subsequent quantification of 11 bile acids (unconjugated, glycine-conjugated) in saliva from healthy volunteers and BE patients by ultra-high-performance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry. Moreover, high resolution MS (Orbitrap-MS) was utilized for identification of new bile acids in saliva. Methods for saliva collection in-cluding simple spitting and the Salivette (R) saliva collection system were compared; the latter was found to be unsuitable due to excessive retention of bile acids in the cotton swab. Methanol with 0.1% formic acid were selected for protein precipitation and bile acid extraction prior to SPE. Separation was per-formed in gradient elution of methanol and 0.1% formic acid in less than 10 min. Saliva from BE patients contained higher levels of almost all bile acids, and the tested groups could be distinguished by princi-pal component analysis. In untargeted analysis by high resolution MS, taurine-conjugated bile acids and glycine-conjugated dihydroxy-bile acid sulfate were identified in saliva from healthy volunteers. We pro-pose that analysis of salivary bile acids including taurine conjugates could be applicable in diagnostics of BE, following a larger clinical study.