J 2022

Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?

DOSEDĚLOVÁ, Věra, Marketa LASTOVICKOVA, Juan F AYALA-CABRERA, Jiří DOLINA, Štefan KONEČNÝ et. al.

Basic information

Original name

Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?

Authors

DOSEDĚLOVÁ, Věra (203 Czech Republic, belonging to the institution), Marketa LASTOVICKOVA (203 Czech Republic), Juan F AYALA-CABRERA, Jiří DOLINA (203 Czech Republic, belonging to the institution), Štefan KONEČNÝ (703 Slovakia, belonging to the institution), Oliver J. SCHMITZ and Petr KUBÁŇ (203 Czech Republic)

Edition

Journal of Chromatography A, Amsterdam, ELSEVIER SCIENCE BV, 2022, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30219 Gastroenterology and hepatology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.100

RIV identification code

RIV/00216224:14740/22:00128067

Organization unit

Central European Institute of Technology

DOI

UT WoS

000834927200006

Keywords in English

Barrett’s esophagus; Bileacids; LC-MS; Salivaanalysis; Solid-phaseextraction

Tags

Tags

International impact, Reviewed
Změněno: 14/2/2023 10:00, Mgr. Tereza Miškechová

Abstract

V originále

Bile acids are a group of steroid compounds essential for lipid digestion. However, when bile acids are refluxed into the stomach and the esophagus, during the so called duodenogastroesophageal reflux, they can have a detrimental effect on the esophageal epithelium and cause pathological changes of esophageal tissue, e.g., Barrett's esophagus (BE). The levels of bile acids in saliva could therefore serve as possi-ble biomarkers for the diagnostics of BE. In this work, we focused on optimization of sample collection and preparation by solid-phase extraction and subsequent quantification of 11 bile acids (unconjugated, glycine-conjugated) in saliva from healthy volunteers and BE patients by ultra-high-performance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry. Moreover, high resolution MS (Orbitrap-MS) was utilized for identification of new bile acids in saliva. Methods for saliva collection in-cluding simple spitting and the Salivette (R) saliva collection system were compared; the latter was found to be unsuitable due to excessive retention of bile acids in the cotton swab. Methanol with 0.1% formic acid were selected for protein precipitation and bile acid extraction prior to SPE. Separation was per-formed in gradient elution of methanol and 0.1% formic acid in less than 10 min. Saliva from BE patients contained higher levels of almost all bile acids, and the tested groups could be distinguished by princi-pal component analysis. In untargeted analysis by high resolution MS, taurine-conjugated bile acids and glycine-conjugated dihydroxy-bile acid sulfate were identified in saliva from healthy volunteers. We pro-pose that analysis of salivary bile acids including taurine conjugates could be applicable in diagnostics of BE, following a larger clinical study.