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@article{2247520, author = {Dosedělová, Věra and Lastovickova, Marketa and AyalaandCabrera, Juan F and Dolina, Jiří and Konečný, Štefan and Schmitz, Oliver J. and Kubáň, Petr}, article_location = {Amsterdam}, article_number = {August 2022}, doi = {http://dx.doi.org/10.1016/j.chroma.2022.463287}, keywords = {Barrett’s esophagus; Bileacids; LC-MS; Salivaanalysis; Solid-phaseextraction}, language = {eng}, issn = {0021-9673}, journal = {Journal of Chromatography A}, title = {Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?}, url = {https://www.sciencedirect.com/science/article/pii/S0021967322004800?via%3Dihub#keys0001}, volume = {1676}, year = {2022} }
TY - JOUR ID - 2247520 AU - Dosedělová, Věra - Lastovickova, Marketa - Ayala-Cabrera, Juan F - Dolina, Jiří - Konečný, Štefan - Schmitz, Oliver J. - Kubáň, Petr PY - 2022 TI - Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus? JF - Journal of Chromatography A VL - 1676 IS - August 2022 SP - 1-13 EP - 1-13 PB - ELSEVIER SCIENCE BV SN - 00219673 KW - Barrett’s esophagus KW - Bileacids KW - LC-MS KW - Salivaanalysis KW - Solid-phaseextraction UR - https://www.sciencedirect.com/science/article/pii/S0021967322004800?via%3Dihub#keys0001 N2 - Bile acids are a group of steroid compounds essential for lipid digestion. However, when bile acids are refluxed into the stomach and the esophagus, during the so called duodenogastroesophageal reflux, they can have a detrimental effect on the esophageal epithelium and cause pathological changes of esophageal tissue, e.g., Barrett's esophagus (BE). The levels of bile acids in saliva could therefore serve as possi-ble biomarkers for the diagnostics of BE. In this work, we focused on optimization of sample collection and preparation by solid-phase extraction and subsequent quantification of 11 bile acids (unconjugated, glycine-conjugated) in saliva from healthy volunteers and BE patients by ultra-high-performance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry. Moreover, high resolution MS (Orbitrap-MS) was utilized for identification of new bile acids in saliva. Methods for saliva collection in-cluding simple spitting and the Salivette (R) saliva collection system were compared; the latter was found to be unsuitable due to excessive retention of bile acids in the cotton swab. Methanol with 0.1% formic acid were selected for protein precipitation and bile acid extraction prior to SPE. Separation was per-formed in gradient elution of methanol and 0.1% formic acid in less than 10 min. Saliva from BE patients contained higher levels of almost all bile acids, and the tested groups could be distinguished by princi-pal component analysis. In untargeted analysis by high resolution MS, taurine-conjugated bile acids and glycine-conjugated dihydroxy-bile acid sulfate were identified in saliva from healthy volunteers. We pro-pose that analysis of salivary bile acids including taurine conjugates could be applicable in diagnostics of BE, following a larger clinical study. ER -
DOSEDĚLOVÁ, Věra, Marketa LASTOVICKOVA, Juan F AYALA-CABRERA, Jiří DOLINA, Štefan KONEČNÝ, Oliver J. SCHMITZ and Petr KUBÁŇ. Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus? \textit{Journal of Chromatography A}. Amsterdam: ELSEVIER SCIENCE BV, 2022, vol.~1676, August 2022, p.~1-13. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2022.463287.
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