HU, Yaping, Petr ŠMARDA, Ganping LIU, Beibei WANG, Xiaoge GAO and Qirong GUO. High-depth transcriptome reveals differences in natural haploid Ginkgo biloba L. due to the effect of reduced gene dosage. International Journal of Molecular Sciences. MDPI, 2022, vol. 23, No 16, p. 1-16. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms23168958.
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Basic information
Original name High-depth transcriptome reveals differences in natural haploid Ginkgo biloba L. due to the effect of reduced gene dosage
Authors HU, Yaping (156 China, belonging to the institution), Petr ŠMARDA (203 Czech Republic, belonging to the institution), Ganping LIU (156 China), Beibei WANG (156 China), Xiaoge GAO (156 China) and Qirong GUO (156 China, guarantor).
Edition International Journal of Molecular Sciences, MDPI, 2022, 1422-0067.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10611 Plant sciences, botany
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW odkaz na článek na stránkách vydavatelství
Impact factor Impact factor: 5.600
RIV identification code RIV/00216224:14310/22:00128068
Organization unit Faculty of Science
Doi http://dx.doi.org/10.3390/ijms23168958
UT WoS 000845826100001
Keywords in English ginkgo; haploid; transcriptome; gene dosage; mechanism
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 25/1/2023 12:17.
Abstract
As a representative of gymnosperms, the discovery of natural haploids of Ginkgo biloba L. has opened a new door for its research. Haploid germplasm has always been a research material of interest to researchers because of its special characteristics. However, we do not yet know the special features and mechanisms of haploid ginkgo following this significant discovery. In this study, we conducted a homogenous garden experiment on haploid and diploid ginkgo to explore the differences in growth, physiology and biochemistry between the two. Additionally, a high-depth transcriptome database of both was established to reveal their transcriptional differences. The results showed that haploid ginkgo exhibited weaker growth potential, lower photosynthesis and flavonoid accumulation capacity. Although the up-regulated expression of DEGs in haploid ginkgo reached 46.7% of the total DEGs in the whole transcriptome data, the gene sets of photosynthesis metabolic, glycolysis/gluconeogenesis and flavonoid biosynthesis pathways, which were significantly related to these differences, were found to show a significant down-regulated expression trend by gene set enrichment analysis (GSEA). We further found that the major metabolic pathways in the haploid ginkgo transcriptional database were down-regulated in expression compared to the diploid. This study reveals for the first time the phenotypic, growth and physiological differences in haploid ginkgos, and demonstrates their transcriptional patterns based on high-depth transcriptomic data, laying the foundation for subsequent in-depth studies of haploid ginkgos.
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