Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing

@article{2247538,
   author = {Škovranová, Gabriela and Čulenová, Marie and Treml, Jakub and Dzuricka, Lucia and Marova, Ivana and Sychrová, Alice},
   article_location = {Lausanne},
   article_number = {November},
   doi = {http://dx.doi.org/10.3389/fphar.2022.1068371},
   keywords = {antibacterial activity; antimicrobial resistance; kuwanon C; MRSA; mulberry; prenylated phenolics; synergy; wound healing},
   language = {eng},
   issn = {1663-9812},
   journal = {Frontiers in Pharmacology},
   title = {Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing},
   url = {https://www.frontiersin.org/articles/10.3389/fphar.2022.1068371/full},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2247538
AU  - Škovranová, Gabriela - Čulenová, Marie - Treml, Jakub - Dzuricka, Lucia - Marova, Ivana - Sychrová, Alice
PY  - 2022
TI  - Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing
JF  - Frontiers in Pharmacology
VL  - 13
IS  - November
SP  - 1-13
EP  - 1-13
PB  - Frontiers Media SA
SN  - 16639812
KW  - antibacterial activity
KW  - antimicrobial resistance
KW  - kuwanon C
KW  - MRSA
KW  - mulberry
KW  - prenylated phenolics
KW  - synergy
KW  - wound healing
UR  - https://www.frontiersin.org/articles/10.3389/fphar.2022.1068371/full
N2  - Antimicrobial resistance is a public health threat and the increasing number of multidrug-resistant bacteria is a major concern worldwide. Common antibiotics are becoming ineffective for skin infections and wounds, making the search for new therapeutic options increasingly urgent. The present study aimed to investigate the antibacterial potential of prenylated phenolics in wound healing. Phenolic compounds isolated from the root bark of Morus alba L. were investigated for their antistaphylococcal potential both alone and in combination with commonly used antibiotics. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by microdilution and agar method. Synergy was investigated using the checkerboard titration technique. Membrane-disrupting activity and efflux pump inhibition were evaluated to describe the potentiating effect. Prenylated phenolics inhibited bacterial growth of methicillin-resistant Staphylococcus aureus (MRSA) at lower concentrations (MIC 2-8 mu g/ml) than commonly used antibiotics. The combination of active phenolics with kanamycin, oxacillin, and ciprofloxacin resulted in a decrease in the MIC of the antimicrobial agent. Kuwanon C, E, T, morusin, and albafuran C showed synergy (FICi 0.375-0.5) with oxacillin and/or kanamycin. Prenylated phenolics disrupted membrane permeability statistically significantly (from 28 +/- 16.48% up to 73 +/- 2.83%), and membrane disruption contributes to the complex antibacterial activity against MRSA. In addition, kuwanon C could be considered an efflux pump inhibitor. Despite the antibacterial effect on MRSA and the multiple biological activities, the prenylated phenolics at microbially significant concentrations have a minor effect on human keratinocyte (HaCaT) viability. In conclusion, prenylated phenolics in combination with commonly used antibiotics are promising candidates for the treatment of MRSA infections and wound healing, although further studies are needed.
ER  - 

ŠKOVRANOVÁ, Gabriela, Marie ČULENOVÁ, Jakub TREML, Lucia DZURICKA, Ivana MAROVA and Alice SYCHROVÁ. Prenylated phenolics from Morus alba against MRSA infections as a strategy for wound healing. \textit{Frontiers in Pharmacology}. Lausanne: Frontiers Media SA, 2022, vol.~13, November, p.~1-13. ISSN~1663-9812. Available from: https://dx.doi.org/10.3389/fphar.2022.1068371.