Vaccine against Streptococcus suis Infection in Pig Based on
Alternative Carrier Protein Conjugate

J 2022

Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate

KRÁLOVÁ, Natálie; Hana STEPANOVA; Jan GEBAUER; Adam NOREK; Katarina MATIASKOVA et al.

Základní údaje

Originální název

Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate

Autoři

Vydání

Vaccines, MDPI, 2022, 2076-393X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.800

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00128096

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Streptococcus suis; conjugate vaccine; OVA; CRM197; pig

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 1. 2023 13:26, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Streptococcus suis is a serious pathogen in the pig industry with zoonotic potential. With respect to the current effort to reduce antibiotic use in animals, a prophylactic measure is needed to control the disease burden. Unfortunately, immunization against streptococcal pathogens is challenging due to nature of the interaction between the pathogen and the host immune system, but vaccines based on conjugates of capsular polysaccharide (CPS) and carrier protein were proved to be efficient. The main obstacle of these vaccines is manufacturing cost, limiting their use in animals. In this work, we tested an experimental vaccine against Streptococcus suis serotype 2 based on capsular polysaccharide conjugated to chicken ovalbumin (OVA) and compared its immunogenicity and protectivity with a vaccine based on CRM197 conjugate. Ovalbumin was selected as a cheap alternative to recombinant carrier proteins widely used in vaccines for human use. We found that the ovalbumin-based experimental vaccine successfully induced immune response in pigs, and the IgG antibody response was even higher than after immunization with capsular polysaccharide-CRM197 conjugate. Protectivity of vaccination against infection was evaluated in the challenge experiment and was found promising for both conjugates.

Citovat

KRÁLOVÁ, Natálie; Hana STEPANOVA; Jan GEBAUER; Adam NOREK; Katarina MATIASKOVA; Monika ZOUHAROVA; Katerina NEDBALCOVA; Vladimir BABAK; Rea JAROSOVA; Peter MAKOVICKY; Ivana KUCHAROVICOVA; Bronislav SIMEK; Hana PLODKOVA; Tomas PECKA a Jan MATIASOVIC. Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate. Vaccines. MDPI, 2022, roč. 10, č. 10, s. 1-18. ISSN 2076-393X. Dostupné z: https://doi.org/10.3390/vaccines10101620.

@article{2247607,
   author = {Králová, Natálie and Stepanova, Hana and Gebauer, Jan and Norek, Adam and Matiaskova, Katarina and Zouharova, Monika and Nedbalcova, Katerina and Babak, Vladimir and Jarosova, Rea and Makovicky, Peter and Kucharovicova, Ivana and Simek, Bronislav and Plodkova, Hana and Pecka, Tomas and Matiasovic, Jan},
   article_number = {10},
   doi = {https://doi.org/10.3390/vaccines10101620},
   keywords = {Streptococcus suis; conjugate vaccine; OVA; CRM197; pig},
   language = {eng},
   issn = {2076-393X},
   journal = {Vaccines},
   title = {Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate},
   url = {https://www.mdpi.com/2076-393X/10/10/1620},
   volume = {10},
   year = {2022}
}

TY  - JOUR
ID  - 2247607
AU  - Králová, Natálie - Stepanova, Hana - Gebauer, Jan - Norek, Adam - Matiaskova, Katarina - Zouharova, Monika - Nedbalcova, Katerina - Babak, Vladimir - Jarosova, Rea - Makovicky, Peter - Kucharovicova, Ivana - Simek, Bronislav - Plodkova, Hana - Pecka, Tomas - Matiasovic, Jan
PY  - 2022
TI  - Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate
JF  - Vaccines
VL  - 10
IS  - 10
SP  - 1-18
EP  - 1-18
PB  - MDPI
SN  - 2076393X
KW  - Streptococcus suis
KW  - conjugate vaccine
KW  - OVA
KW  - CRM197
KW  - pig
UR  - https://www.mdpi.com/2076-393X/10/10/1620
N2  - Streptococcus suis is a serious pathogen in the pig industry with zoonotic potential. With respect to the current effort to reduce antibiotic use in animals, a prophylactic measure is needed to control the disease burden. Unfortunately, immunization against streptococcal pathogens is challenging due to nature of the interaction between the pathogen and the host immune system, but vaccines based on conjugates of capsular polysaccharide (CPS) and carrier protein were proved to be efficient. The main obstacle of these vaccines is manufacturing cost, limiting their use in animals. In this work, we tested an experimental vaccine against Streptococcus suis serotype 2 based on capsular polysaccharide conjugated to chicken ovalbumin (OVA) and compared its immunogenicity and protectivity with a vaccine based on CRM197 conjugate. Ovalbumin was selected as a cheap alternative to recombinant carrier proteins widely used in vaccines for human use. We found that the ovalbumin-based experimental vaccine successfully induced immune response in pigs, and the IgG antibody response was even higher than after immunization with capsular polysaccharide-CRM197 conjugate. Protectivity of vaccination against infection was evaluated in the challenge experiment and was found promising for both conjugates.
ER  -

KRÁLOVÁ, Natálie; Hana STEPANOVA; Jan GEBAUER; Adam NOREK; Katarina MATIASKOVA; Monika ZOUHAROVA; Katerina NEDBALCOVA; Vladimir BABAK; Rea JAROSOVA; Peter MAKOVICKY; Ivana KUCHAROVICOVA; Bronislav SIMEK; Hana PLODKOVA; Tomas PECKA a Jan MATIASOVIC. Vaccine against Streptococcus suis Infection in Pig Based on Alternative Carrier Protein Conjugate. \textit{Vaccines}. MDPI, 2022, roč.~10, č.~10, s.~1-18. ISSN~2076-393X. Dostupné z: https://doi.org/10.3390/vaccines10101620.

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