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@article{224771, author = {Juškaitis, Rimas and Wilson, Tony and Neil, Mark and Kozubek, Michal}, article_number = {6603}, keywords = {confocal microscopy; SCAM}, language = {eng}, issn = {0028-0836}, journal = {Nature}, title = {Efficient real-time confocal microscopy with white light sources}, volume = {383}, year = {1996} }
TY - JOUR ID - 224771 AU - Juškaitis, Rimas - Wilson, Tony - Neil, Mark - Kozubek, Michal PY - 1996 TI - Efficient real-time confocal microscopy with white light sources JF - Nature VL - 383 IS - 6603 SP - 804 EP - 804 SN - 00280836 KW - confocal microscopy KW - SCAM N2 - The main advantage of confocal microscopes over their conventional counterparts arises from their ability to optically 'section' nearly transparent materials; the thin image slices thus obtained can be used to reconstruct three-dimensional images, a capability which is particularly useful for the study of biological specimens. Confocal microscopes have previously used either a single laser-illuminated point-source and single point-detector (which are scanned in tandem across the object) or white-light illumination with multiple point-sources and detectors. Single-point-source systems, however, do not usually form images in real time and are restricted to using available laser wavelengths. Multiple-point-source systems, on the other hand, produce images in real time but use light very inefficiently - typically 1% or less is used for imaging. Here we demonstrate a white-light, multiple-point-source method which can in principle produce images in real time with light efficiencies as high as 50%. This system is likely to find broad practical application, particularly in the imaging of weakly reflecting or weakly fluorescent specimens. ER -
JUŠKAITIS, Rimas, Tony WILSON, Mark NEIL and Michal KOZUBEK. Efficient real-time confocal microscopy with white light sources. \textit{Nature}. 1996, vol.~383, No~6603, p.~804-806. ISSN~0028-0836.
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