JUŠKAITIS, Rimas, Tony WILSON, Mark NEIL and Michal KOZUBEK. Efficient real-time confocal microscopy with white light sources. Nature. vol. 383, No 6603, p. 804-806. ISSN 0028-0836. 1996.
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Basic information
Original name Efficient real-time confocal microscopy with white light sources
Authors JUŠKAITIS, Rimas, Tony WILSON, Mark NEIL and Michal KOZUBEK (203 Czech Republic).
Edition Nature, 1996, 0028-0836.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10306 Optics
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14330/96:00001521
Organization unit Faculty of Informatics
Keywords in English confocal microscopy; SCAM
Tags cbia-web, confocal microscopy, SCAM
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Michal Kozubek, Ph.D., učo 3740. Changed: 7/5/2010 17:26.
Abstract
The main advantage of confocal microscopes over their conventional counterparts arises from their ability to optically 'section' nearly transparent materials; the thin image slices thus obtained can be used to reconstruct three-dimensional images, a capability which is particularly useful for the study of biological specimens. Confocal microscopes have previously used either a single laser-illuminated point-source and single point-detector (which are scanned in tandem across the object) or white-light illumination with multiple point-sources and detectors. Single-point-source systems, however, do not usually form images in real time and are restricted to using available laser wavelengths. Multiple-point-source systems, on the other hand, produce images in real time but use light very inefficiently - typically 1% or less is used for imaging. Here we demonstrate a white-light, multiple-point-source method which can in principle produce images in real time with light efficiencies as high as 50%. This system is likely to find broad practical application, particularly in the imaging of weakly reflecting or weakly fluorescent specimens.
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