J 1996

Efficient real-time confocal microscopy with white light sources

JUŠKAITIS, Rimas, Tony WILSON, Mark NEIL and Michal KOZUBEK

Basic information

Original name

Efficient real-time confocal microscopy with white light sources

Authors

JUŠKAITIS, Rimas, Tony WILSON, Mark NEIL and Michal KOZUBEK (203 Czech Republic)

Edition

Nature, 1996, 0028-0836

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10306 Optics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/96:00001521

Organization unit

Faculty of Informatics

Keywords in English

confocal microscopy; SCAM

Tags

Tags

International impact, Reviewed
Změněno: 7/5/2010 17:26, prof. RNDr. Michal Kozubek, Ph.D.

Abstract

V originále

The main advantage of confocal microscopes over their conventional counterparts arises from their ability to optically 'section' nearly transparent materials; the thin image slices thus obtained can be used to reconstruct three-dimensional images, a capability which is particularly useful for the study of biological specimens. Confocal microscopes have previously used either a single laser-illuminated point-source and single point-detector (which are scanned in tandem across the object) or white-light illumination with multiple point-sources and detectors. Single-point-source systems, however, do not usually form images in real time and are restricted to using available laser wavelengths. Multiple-point-source systems, on the other hand, produce images in real time but use light very inefficiently - typically 1% or less is used for imaging. Here we demonstrate a white-light, multiple-point-source method which can in principle produce images in real time with light efficiencies as high as 50%. This system is likely to find broad practical application, particularly in the imaging of weakly reflecting or weakly fluorescent specimens.