Further data on the distribution of Dirofilaria spp. in the
Czech Republic in dogs

J 2022

Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs

JURANKOVA, Jana, Barbora Senkyrikova MITKOVA, Marcela NOVOTNA, Lada HOFMANNOVA, Barbora CERVENA et. al.

Basic information

Original name

Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs

Authors

JURANKOVA, Jana (guarantor), Barbora Senkyrikova MITKOVA, Marcela NOVOTNA, Lada HOFMANNOVA, Barbora CERVENA, Dwight D BOWMAN and David MODRÝ (203 Czech Republic, belonging to the institution)

Edition

Folia Parasitologica, AV ČR, Institute of Parasitology, Biology Centrum, 2022, 0015-5683

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30310 Parasitology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.600

RIV identification code

RIV/00216224:14310/22:00128113

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.14411/fp.2022.007

UT WoS

000779883000001

Keywords in English

dirofilariosis; Central Europe; autochthonous infection; COI

Abstract

V originále

Based on previously published data, the Czech Republic is regarded an endemic country of the onchocercid nematodes Dirofilaria immitis (Leidy, 1856) and Dirofilaria repens Railliet et Henry, 1911. Nevertheless, while cases of D. repens are commonly reported from dogs in South Moravia, no recent records of D. immitis are available. Therefore, the present study was performed to clarify the occurrence of both species ofDirofilaria Railliet et Henry, 1910. Blood samples of 551 dogs sampled during 2015 and 2016 were analysed microscopically for presence of microfilariae and blood sera were examined by IDEXX SNAP (R) 4Dx (R) test (IDEXX, USA). DNA from blood of microscopically positive dogs was extracted and PCR protocol amplifying fragment of cytochrome c oxi-dase I (COI) gene was performed; PCR products were then sequenced. All dogs from the Bohemian part of the Czech Republic were negative. The prevalence of D. repens in the Moravian region was 5.7% (27/476). BLAST analyses of obtained sequences confirmed the presence of D. repens (99-100% identical to KX265049). All sampled animals showed a negative result for D. immitis antigen in IDEXX SNAP (R) 4Dx (R) test. Our study confirmed the previously reported occurrence of D. repens in South Moravia and revealed its spreading from the epicentre to the north and west. PCR with subsequent sequencing together with negative results for D. immitis antigen in IDEXX SNAP (R) 4Dx (R) test revealed only D. repens infection. A previously published autochthonous infection of dogs with D. immitis in South Moravia was not confirmed.

Citovat

JURANKOVA, Jana, Barbora Senkyrikova MITKOVA, Marcela NOVOTNA, Lada HOFMANNOVA, Barbora CERVENA, Dwight D BOWMAN and David MODRÝ. Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs. Folia Parasitologica. AV ČR, Institute of Parasitology, Biology Centrum, 2022, vol. 69, MAR, p. "007", 5 pp. ISSN 0015-5683. Available from: https://dx.doi.org/10.14411/fp.2022.007.

@article{2247800,
   author = {Jurankova, Jana and Mitkova, Barbora Senkyrikova and Novotna, Marcela and Hofmannova, Lada and Cervena, Barbora and Bowman, Dwight D and Modrý, David},
   article_number = {MAR},
   doi = {http://dx.doi.org/10.14411/fp.2022.007},
   keywords = {dirofilariosis; Central Europe; autochthonous infection; COI},
   language = {eng},
   issn = {0015-5683},
   journal = {Folia Parasitologica},
   title = {Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs},
   url = {https://doi.org/10.14411/fp.2022.007},
   volume = {69},
   year = {2022}
}

TY  - JOUR
ID  - 2247800
AU  - Jurankova, Jana - Mitkova, Barbora Senkyrikova - Novotna, Marcela - Hofmannova, Lada - Cervena, Barbora - Bowman, Dwight D - Modrý, David
PY  - 2022
TI  - Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs
JF  - Folia Parasitologica
VL  - 69
IS  - MAR
SP  - "007"
EP  - "007"
PB  - AV ČR, Institute of Parasitology, Biology Centrum
SN  - 00155683
KW  - dirofilariosis
KW  - Central Europe
KW  - autochthonous infection
KW  - COI
UR  - https://doi.org/10.14411/fp.2022.007
N2  - Based on previously published data, the Czech Republic is regarded an endemic country of the onchocercid nematodes Dirofilaria immitis (Leidy, 1856) and Dirofilaria repens Railliet et Henry, 1911. Nevertheless, while cases of D. repens are commonly reported from dogs in South Moravia, no recent records of D. immitis are available. Therefore, the present study was performed to clarify the occurrence of both species ofDirofilaria Railliet et Henry, 1910. Blood samples of 551 dogs sampled during 2015 and 2016 were analysed microscopically for presence of microfilariae and blood sera were examined by IDEXX SNAP (R) 4Dx (R) test (IDEXX, USA). DNA from blood of microscopically positive dogs was extracted and PCR protocol amplifying fragment of cytochrome c oxi-dase I (COI) gene was performed; PCR products were then sequenced. All dogs from the Bohemian part of the Czech Republic were negative. The prevalence of D. repens in the Moravian region was 5.7% (27/476). BLAST analyses of obtained sequences confirmed the presence of D. repens (99-100% identical to KX265049). All sampled animals showed a negative result for D. immitis antigen in IDEXX SNAP (R) 4Dx (R) test. Our study confirmed the previously reported occurrence of D. repens in South Moravia and revealed its spreading from the epicentre to the north and west. PCR with subsequent sequencing together with negative results for D. immitis antigen in IDEXX SNAP (R) 4Dx (R) test revealed only D. repens infection. A previously published autochthonous infection of dogs with D. immitis in South Moravia was not confirmed.
ER  -

JURANKOVA, Jana, Barbora Senkyrikova MITKOVA, Marcela NOVOTNA, Lada HOFMANNOVA, Barbora CERVENA, Dwight D BOWMAN and David MODRÝ. Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs. \textit{Folia Parasitologica}. AV ČR, Institute of Parasitology, Biology Centrum, 2022, vol.~69, MAR, p.~''007'', 5 pp. ISSN~0015-5683. Available from: https://dx.doi.org/10.14411/fp.2022.007.

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