Other formats:
BibTeX
LaTeX
RIS
@article{2248041, author = {Trivedi, Shalini and Blažíčková, Jitka and Silva, Nicola}, article_location = {Oxford}, article_number = {21}, doi = {http://dx.doi.org/10.1093/nar/gkac1153}, keywords = {PARG; BRCA1–BARD1; DNA repair pathway choice; gametogenesis}, language = {eng}, issn = {0305-1048}, journal = {Nucleic acids research}, title = {PARG and BRCA1-BARD1 cooperative function regulates DNA repair pathway choice during gametogenesis}, url = {https://academic.oup.com/nar/article/50/21/12291/6882108?login=true}, volume = {50}, year = {2022} }
TY - JOUR ID - 2248041 AU - Trivedi, Shalini - Blažíčková, Jitka - Silva, Nicola PY - 2022 TI - PARG and BRCA1-BARD1 cooperative function regulates DNA repair pathway choice during gametogenesis JF - Nucleic acids research VL - 50 IS - 21 SP - 12291-12308 EP - 12291-12308 PB - Oxford University Press SN - 03051048 KW - PARG KW - BRCA1–BARD1 KW - DNA repair pathway choice KW - gametogenesis UR - https://academic.oup.com/nar/article/50/21/12291/6882108?login=true N2 - Meiotic chromosome segregation relies on programmed DNA double-strand break induction. These are in turn repaired by homologous recombination, generating physical attachments between the parental chromosomes called crossovers. A subset of breaks yields recombinant outcomes, while crossover-independent mechanisms repair the majority of lesions. The balance between different repair pathways is crucial to ensure genome integrity. We show that Caenorhabditis elegans BRC-1/BRCA1-BRD-1/BARD1 and PARG-1/PARG form a complex in vivo, essential for accurate DNA repair in the germline. Simultaneous depletion of BRC-1 and PARG-1 causes synthetic lethality due to reduced crossover formation and impaired break repair, evidenced by hindered RPA-1 removal and presence of aberrant chromatin bodies in diakinesis nuclei, whose formation depends on spo-11 function. These factors undergo a similar yet independent loading in developing oocytes, consistent with operating in different pathways. Abrogation of KU- or Theta-mediated end joining elicits opposite effects in brc-1; parg-1 doubles, suggesting a profound impact in influencing DNA repair pathway choice by BRC-1-PARG-1. Importantly, lack of PARG-1 catalytic activity suppresses untimely accumulation of RAD-51 foci in brc-1 mutants but is only partially required for fertility. Our data show that BRC-1/BRD-1-PARG-1 joint function is essential for genome integrity in meiotic cells by regulating multiple DNA repair pathways. ER -
TRIVEDI, Shalini, Jitka BLAŽÍČKOVÁ and Nicola SILVA. PARG and BRCA1-BARD1 cooperative function regulates DNA repair pathway choice during gametogenesis. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2022, vol.~50, No~21, p.~12291-12308. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkac1153.
|