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@article{2248093,
author = {Das, Debabrata and Trivedi, Shalini and Blažíčková, Jitka and Arur, Swathi and Silva, Nicola},
article_location = {CARY},
article_number = {5},
doi = {http://dx.doi.org/10.1093/g3journal/jkac079},
keywords = {Caenorhabditis elegans meiosis; HORMA-domain proteins; HTP-3},
language = {eng},
issn = {2160-1836},
journal = {G3-Genes, Genomes, Genetics},
title = {Phosphorylation of HORMA-domain protein HTP-3 at Serine 285 is dispensable for crossover formation},
url = {https://academic.oup.com/g3journal/article/12/5/jkac079/6564663?login=true},
volume = {12},
year = {2022}
}
TY - JOUR
ID - 2248093
AU - Das, Debabrata - Trivedi, Shalini - Blažíčková, Jitka - Arur, Swathi - Silva, Nicola
PY - 2022
TI - Phosphorylation of HORMA-domain protein HTP-3 at Serine 285 is dispensable for crossover formation
JF - G3-Genes, Genomes, Genetics
VL - 12
IS - 5
SP - 1-10
EP - 1-10
PB - OXFORD UNIV PRESS INC
SN - 21601836
KW - Caenorhabditis elegans meiosis
KW - HORMA-domain proteins
KW - HTP-3
UR - https://academic.oup.com/g3journal/article/12/5/jkac079/6564663?login=true
N2 - Generation of functional gametes is accomplished through a multilayered and finely orchestrated succession of events during meiotic progression. In the Caenorhabditis elegans germline, the HORMA-domain-containing protein HTP-3 plays pivotal roles for the establishment of chromosome axes and the efficient induction of programmed DNA double-strand breaks, both of which are crucial for crossover formation. Double-strand breaks allow for accurate chromosome segregation during the first meiotic division and therefore are an essential requirement for the production of healthy gametes. Phosphorylation-dependent regulation of HORMAD protein plays important roles in controlling meiotic chromosome behavior. Here, we document a phospho-site in HTP-3 at Serine 285 that is constitutively phosphorylated during meiotic prophase I. pHTP-3(S285) localization overlaps with panHTP-3 except in nuclei undergoing physiological apoptosis, in which pHTP-3 is absent. Surprisingly, we observed that phosphorylation of HTP-3 at S285 is independent of the canonical kinases that control meiotic progression in nematodes. During meiosis, the htp-3(S285A) mutant displays accelerated RAD-51 turnover, but no other meiotic abnormalities. Altogether, these data indicate that the Ser285 phosphorylation is independent of canonical meiotic protein kinases and does not regulate HTP-3-dependent meiotic processes. We propose a model wherein phosphorylation of HTP-3 occurs through noncanonical or redundant meiotic kinases and/or is likely redundant with additional phospho-sites for function in vivo.
ER -
DAS, Debabrata, Shalini TRIVEDI, Jitka BLAŽÍČKOVÁ, Swathi ARUR and Nicola SILVA. Phosphorylation of HORMA-domain protein HTP-3 at Serine 285 is dispensable for crossover formation. \textit{G3-Genes, Genomes, Genetics}. CARY: OXFORD UNIV PRESS INC, 2022, vol.~12, No~5, p.~1-10. ISSN~2160-1836. Available from: https://dx.doi.org/10.1093/g3journal/jkac079.
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