Continuous double-strand break induction and their differential
processing sustain chiasma formation during Caenorhabditis
elegans meiosis

J 2022

Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis

HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN et. al.

Basic information

Original name

Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis

Authors

HICKS, Tara, Shalini TRIVEDI (356 India, belonging to the institution), Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE and Nicola SILVA (380 Italy, guarantor, belonging to the institution)

Edition

Cell Reports, CAMBRIDGE, Cell Press, 2022, 2211-1247

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.800

RIV identification code

RIV/00216224:14110/22:00128181

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.celrep.2022.111403

UT WoS

000869019800008

Keywords in English

Caenorhabditis elegans meiosis; double-strand break induction; sustain chiasma formation

Abstract

V originále

Faithful chromosome segregation into gametes depends on Spo11-induced DNA double-strand breaks (DSBs). These yield single-stranded 30 tails upon resection to promote crossovers (COs). While early Mre11-dependent end resection is the predominant pathway in most organisms, Exo1 or Dna2/BLM can also contribute to the efficient processing of meiotic DSBs. Although its enzymatic activity has been thor-oughly dissected, the temporal dynamics underlying Spo11 activity have remained mostly elusive. We show that, in Caenorhabditis elegans, SPO-11-mediated DSB induction takes place throughout early meiotic prophase I until mid-late pachynema. We find that late DSBs are essential for CO formation and are prefer-entially processed by EXO-1 and DNA-2 in a redundant fashion. Further, EXO-1-DNA-2-mediated resection ensures completion of conservative DSB repair and discourages activation of KU-dependent end joining. Taken together, our data unveil important temporal aspects of DSB induction and identify previously un-known functional implications for EXO-1-DNA-2-mediated resection activity in C. elegans.

Links

GA20-08819S, research and development project

Name: Pochopení úlohy PARG při podpoře tvorby a oprav dvouřetězcových zlomů DNA v meióze

Investor: Czech Science Foundation

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE and Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. Cell Reports. CAMBRIDGE: Cell Press, 2022, vol. 40, No 13, p. 1-20. ISSN 2211-1247. Available from: https://dx.doi.org/10.1016/j.celrep.2022.111403.

@article{2248097,
   author = {Hicks, Tara and Trivedi, Shalini and Eppert, Mikayla and Bowman, Richard and Tian, Hui and Dafalla, Amna and Crahan, Caroline and Smolikove, Sarit and Silva, Nicola},
   article_location = {CAMBRIDGE},
   article_number = {13},
   doi = {http://dx.doi.org/10.1016/j.celrep.2022.111403},
   keywords = {Caenorhabditis elegans meiosis; double-strand break induction; sustain chiasma formation},
   language = {eng},
   issn = {2211-1247},
   journal = {Cell Reports},
   title = {Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis},
   url = {https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub},
   volume = {40},
   year = {2022}
}

TY  - JOUR
ID  - 2248097
AU  - Hicks, Tara - Trivedi, Shalini - Eppert, Mikayla - Bowman, Richard - Tian, Hui - Dafalla, Amna - Crahan, Caroline - Smolikove, Sarit - Silva, Nicola
PY  - 2022
TI  - Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis
JF  - Cell Reports
VL  - 40
IS  - 13
SP  - 1-20
EP  - 1-20
PB  - Cell Press
SN  - 22111247
KW  - Caenorhabditis elegans meiosis
KW  - double-strand break induction
KW  - sustain chiasma formation
UR  - https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub
N2  - Faithful chromosome segregation into gametes depends on Spo11-induced DNA double-strand breaks (DSBs). These yield single-stranded 30 tails upon resection to promote crossovers (COs). While early Mre11-dependent end resection is the predominant pathway in most organisms, Exo1 or Dna2/BLM can also contribute to the efficient processing of meiotic DSBs. Although its enzymatic activity has been thor-oughly dissected, the temporal dynamics underlying Spo11 activity have remained mostly elusive. We show that, in Caenorhabditis elegans, SPO-11-mediated DSB induction takes place throughout early meiotic prophase I until mid-late pachynema. We find that late DSBs are essential for CO formation and are prefer-entially processed by EXO-1 and DNA-2 in a redundant fashion. Further, EXO-1-DNA-2-mediated resection ensures completion of conservative DSB repair and discourages activation of KU-dependent end joining. Taken together, our data unveil important temporal aspects of DSB induction and identify previously un-known functional implications for EXO-1-DNA-2-mediated resection activity in C. elegans.
ER  -

HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE and Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. \textit{Cell Reports}. CAMBRIDGE: Cell Press, 2022, vol.~40, No~13, p.~1-20. ISSN~2211-1247. Available from: https://dx.doi.org/10.1016/j.celrep.2022.111403.

Detailed Information on Publication Record