| HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE and Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. Cell Reports. CAMBRIDGE: Cell Press, 2022, vol. 40, No 13, p. 1-20. ISSN 2211-1247. Available from: https://dx.doi.org/10.1016/j.celrep.2022.111403. |
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@article{2248097,
author = {Hicks, Tara and Trivedi, Shalini and Eppert, Mikayla and Bowman, Richard and Tian, Hui and Dafalla, Amna and Crahan, Caroline and Smolikove, Sarit and Silva, Nicola},
article_location = {CAMBRIDGE},
article_number = {13},
doi = {http://dx.doi.org/10.1016/j.celrep.2022.111403},
keywords = {Caenorhabditis elegans meiosis; double-strand break induction; sustain chiasma formation},
language = {eng},
issn = {2211-1247},
journal = {Cell Reports},
title = {Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis},
url = {https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub},
volume = {40},
year = {2022}
}
TY - JOUR
ID - 2248097
AU - Hicks, Tara - Trivedi, Shalini - Eppert, Mikayla - Bowman, Richard - Tian, Hui - Dafalla, Amna - Crahan, Caroline - Smolikove, Sarit - Silva, Nicola
PY - 2022
TI - Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis
JF - Cell Reports
VL - 40
IS - 13
SP - 1-20
EP - 1-20
PB - Cell Press
SN - 22111247
KW - Caenorhabditis elegans meiosis
KW - double-strand break induction
KW - sustain chiasma formation
UR - https://www.sciencedirect.com/science/article/pii/S2211124722012402?via%3Dihub
N2 - Faithful chromosome segregation into gametes depends on Spo11-induced DNA double-strand breaks (DSBs). These yield single-stranded 30 tails upon resection to promote crossovers (COs). While early Mre11-dependent end resection is the predominant pathway in most organisms, Exo1 or Dna2/BLM can also contribute to the efficient processing of meiotic DSBs. Although its enzymatic activity has been thor-oughly dissected, the temporal dynamics underlying Spo11 activity have remained mostly elusive. We show that, in Caenorhabditis elegans, SPO-11-mediated DSB induction takes place throughout early meiotic prophase I until mid-late pachynema. We find that late DSBs are essential for CO formation and are prefer-entially processed by EXO-1 and DNA-2 in a redundant fashion. Further, EXO-1-DNA-2-mediated resection ensures completion of conservative DSB repair and discourages activation of KU-dependent end joining. Taken together, our data unveil important temporal aspects of DSB induction and identify previously un-known functional implications for EXO-1-DNA-2-mediated resection activity in C. elegans.
ER -
HICKS, Tara, Shalini TRIVEDI, Mikayla EPPERT, Richard BOWMAN, Hui TIAN, Amna DAFALLA, Caroline CRAHAN, Sarit SMOLIKOVE and Nicola SILVA. Continuous double-strand break induction and their differential processing sustain chiasma formation during Caenorhabditis elegans meiosis. \textit{Cell Reports}. CAMBRIDGE: Cell Press, 2022, vol.~40, No~13, p.~1-20. ISSN~2211-1247. Available from: https://dx.doi.org/10.1016/j.celrep.2022.111403.
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