Leishmania (Sauroleishmania) tarentolae isolation and sympatric
occurrence with Leishmania (Leishmania) infantum in geckoes,
dogs and sand flies

J 2022

Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies

MENDOZA-ROLDAN, Jairo Alfonso, Andrea ZATELLI, Maria Stefania LATROFA, Roberta IATTA, Marcos Antonio BEZERRA-SANTOS et. al.

Basic information

Original name

Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies

Authors

Edition

PLOS NEGLECTED TROPICAL DISEASES, UNITED STATES, PUBLIC LIBRARY SCIENCE, 2022, 1935-2735

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30303 Infectious Diseases

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.800

RIV identification code

RIV/00216224:14310/22:00128185

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1371/journal.pntd.0010650

UT WoS

000909266700001

Keywords in English

Animals; Dogs; Female; Humans; Leishmania infantum; Lizards; Phlebotomus; Polymerase Chain Reaction; Psychodidae

Abstract

V originále

The trypanosomatid protist Leishmania tarentolae is a saurian-associated parasite vectored by the Sergentomyia minuta sand fly. This study aimed to confirm the circulation of L. infantum and L. tarentolae in sand flies, reptiles and dogs and to isolate new strains of these protists. Reptilian and sheltered dog blood samples were collected, and sand flies were captured. Samples were tested for Leishmania spp. using duplex real-time PCR (dqPCR) and real-time PCR (qPCR); the origin of blood meal was identified in engorged sand flies by conventional PCR. The reptilian blood and intestinal content of sand fly females were cultured. Dog sera were tested by IFAT using both Leishmania species. Four Tarentola mauritanica geckoes were molecularly positive for L. infantum or L. tarentolae, with no coinfections; moreover, amastigote-like forms of L. infantum were observed in the bone marrow. 24/294 sand flies scored positive for Leishmania spp. by dqPCR, 21 S. minuta and two Phlebotomus perniciosus were positive for L. tarentolae, while only a single Ph. perniciosus was positive for L. infantum. Blood meal analysis confirmed reptile and dog in S. minuta, dog and human in Ph. perniciosus and dog in Phlebotomus neglectus. Two axenic strains of L. tarentolae were obtained. Twelve of 19 dogs scored positive for L. infantum and L. tarentolae by IFAT and three of them also for L. infantum by dqPCR, and six by qPCR. These data confirm the sympatric circulation of L. infantum and L. tarentolae in geckoes, sand flies, and dogs, and suggest that geckoes may be infected with L. infantum. © 2022 Mendoza-Roldan et al.

Citovat

MENDOZA-ROLDAN, Jairo Alfonso, Andrea ZATELLI, Maria Stefania LATROFA, Roberta IATTA, Marcos Antonio BEZERRA-SANTOS, Giada ANNOSCIA, Floriana GERNONE, Jan VOTÝPKA, David MODRÝ, Lucie TICHÁ, Petr VOLF and Domenico OTRANTO. Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies. PLOS NEGLECTED TROPICAL DISEASES. UNITED STATES: PUBLIC LIBRARY SCIENCE, 2022, vol. 16, No 8, p. 1-16. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0010650.

@article{2248110,
   author = {MendozaandRoldan, Jairo Alfonso and Zatelli, Andrea and Latrofa, Maria Stefania and Iatta, Roberta and BezerraandSantos, Marcos Antonio and Annoscia, Giada and Gernone, Floriana and Votýpka, Jan and Modrý, David and Tichá, Lucie and Volf, Petr and Otranto, Domenico},
   article_location = {UNITED STATES},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0010650},
   keywords = {Animals; Dogs; Female; Humans; Leishmania infantum; Lizards; Phlebotomus; Polymerase Chain Reaction; Psychodidae},
   language = {eng},
   issn = {1935-2735},
   journal = {PLOS NEGLECTED TROPICAL DISEASES},
   title = {Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies},
   url = {https://doi.org/10.1371/journal.pntd.0010650},
   volume = {16},
   year = {2022}
}

TY  - JOUR
ID  - 2248110
AU  - Mendoza-Roldan, Jairo Alfonso - Zatelli, Andrea - Latrofa, Maria Stefania - Iatta, Roberta - Bezerra-Santos, Marcos Antonio - Annoscia, Giada - Gernone, Floriana - Votýpka, Jan - Modrý, David - Tichá, Lucie - Volf, Petr - Otranto, Domenico
PY  - 2022
TI  - Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies
JF  - PLOS NEGLECTED TROPICAL DISEASES
VL  - 16
IS  - 8
SP  - 1-16
EP  - 1-16
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19352735
KW  - Animals
KW  - Dogs
KW  - Female
KW  - Humans
KW  - Leishmania infantum
KW  - Lizards
KW  - Phlebotomus
KW  - Polymerase Chain Reaction
KW  - Psychodidae
UR  - https://doi.org/10.1371/journal.pntd.0010650
N2  - The trypanosomatid protist Leishmania tarentolae is a saurian-associated parasite vectored by the Sergentomyia minuta sand fly. This study aimed to confirm the circulation of L. infantum and L. tarentolae in sand flies, reptiles and dogs and to isolate new strains of these protists. Reptilian and sheltered dog blood samples were collected, and sand flies were captured. Samples were tested for Leishmania spp. using duplex real-time PCR (dqPCR) and real-time PCR (qPCR); the origin of blood meal was identified in engorged sand flies by conventional PCR. The reptilian blood and intestinal content of sand fly females were cultured. Dog sera were tested by IFAT using both Leishmania species. Four Tarentola mauritanica geckoes were molecularly positive for L. infantum or L. tarentolae, with no coinfections; moreover, amastigote-like forms of L. infantum were observed in the bone marrow. 24/294 sand flies scored positive for Leishmania spp. by dqPCR, 21 S. minuta and two Phlebotomus perniciosus were positive for L. tarentolae, while only a single Ph. perniciosus was positive for L. infantum. Blood meal analysis confirmed reptile and dog in S. minuta, dog and human in Ph. perniciosus and dog in Phlebotomus neglectus. Two axenic strains of L. tarentolae were obtained. Twelve of 19 dogs scored positive for L. infantum and L. tarentolae by IFAT and three of them also for L. infantum by dqPCR, and six by qPCR. These data confirm the sympatric circulation of L. infantum and L. tarentolae in geckoes, sand flies, and dogs, and suggest that geckoes may be infected with L. infantum. © 2022 Mendoza-Roldan et al.
ER  -

MENDOZA-ROLDAN, Jairo Alfonso, Andrea ZATELLI, Maria Stefania LATROFA, Roberta IATTA, Marcos Antonio BEZERRA-SANTOS, Giada ANNOSCIA, Floriana GERNONE, Jan VOTÝPKA, David MODRÝ, Lucie TICHÁ, Petr VOLF and Domenico OTRANTO. Leishmania (Sauroleishmania) tarentolae isolation and sympatric occurrence with Leishmania (Leishmania) infantum in geckoes, dogs and sand flies. \textit{PLOS NEGLECTED TROPICAL DISEASES}. UNITED STATES: PUBLIC LIBRARY SCIENCE, 2022, vol.~16, No~8, p.~1-16. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0010650.

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