SCHRADER, Martin, Michal KOZUBEK, Stefan HELL and Tony WILSON. Optical transfer functions of 4Pi confocal microscopes : theory and experiment. Optics Letters. 1997, vol. 22, No 7, p. 436-438. ISSN 0146-9592.
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Basic information
Original name Optical transfer functions of 4Pi confocal microscopes : theory and experiment
Authors SCHRADER, Martin, Michal KOZUBEK (203 Czech Republic), Stefan HELL and Tony WILSON.
Edition Optics Letters, 1997, 0146-9592.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10306 Optics
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.487
RIV identification code RIV/00216224:14330/97:00001526
Organization unit Faculty of Informatics
Keywords in English confocal microscopy; 4Pi; OTF
Tags 4Pi, cbia-web, confocal microscopy, OTF
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Michal Kozubek, Ph.D., učo 3740. Changed: 7/5/2010 17:25.
Abstract
We measure the point-spread function in the two main configurations of 4Pi confocal microscopy as well as in the traditional confocal arrangement and derive the optical transfer functions from the experimental data. The optical transfer functions are in good agreement with their theoretical counterparts. We find a 3.5- to 5-fold increased axial bandwidth of the 4Pi confocal microscope and hence confirm the enhanced spatial-frequency content of 4Pi images.
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