J 1997

Optical transfer functions of 4Pi confocal microscopes : theory and experiment

SCHRADER, Martin, Michal KOZUBEK, Stefan HELL and Tony WILSON

Basic information

Original name

Optical transfer functions of 4Pi confocal microscopes : theory and experiment

Authors

SCHRADER, Martin, Michal KOZUBEK (203 Czech Republic), Stefan HELL and Tony WILSON

Edition

Optics Letters, 1997, 0146-9592

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10306 Optics

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.487

RIV identification code

RIV/00216224:14330/97:00001526

Organization unit

Faculty of Informatics

Keywords in English

confocal microscopy; 4Pi; OTF

Tags

Tags

International impact, Reviewed
Změněno: 7/5/2010 17:25, prof. RNDr. Michal Kozubek, Ph.D.

Abstract

V originále

We measure the point-spread function in the two main configurations of 4Pi confocal microscopy as well as in the traditional confocal arrangement and derive the optical transfer functions from the experimental data. The optical transfer functions are in good agreement with their theoretical counterparts. We find a 3.5- to 5-fold increased axial bandwidth of the 4Pi confocal microscope and hence confirm the enhanced spatial-frequency content of 4Pi images.