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@article{2249820,
author = {Hrbac, Tomas and Kopková, Alena and Siegl, František and Večeřa, Marek and Ručková, Michaela and Kazda, Tomáš and Jančálek, Radim and Hendrych, Michal and Hermanová, Markéta and Vybíhal, Václav and Fadrus, Pavel and Smrčka, Martin and Sokol, Filip and Kubeš, Václav and Lipina, Radim and Slabý, Ondřej and Křen, Leoš and Šána, Jiří},
article_location = {ATHENS},
article_number = {2},
doi = {http://dx.doi.org/10.21873/cgp.20311},
keywords = {Glioblastoma; non-classical human leukocyte antigens; HLA-E; HLA-F; ionizing radiation; prognosis},
language = {eng},
issn = {1109-6535},
journal = {CANCER GENOMICS & PROTEOMICS},
title = {HLA-E and HLA-F Are Overexpressed in Glioblastoma and HLA-E Increased After Exposure to Ionizing Radiation},
url = {https://cgp.iiarjournals.org/content/19/2/151.long},
volume = {19},
year = {2022}
}
TY - JOUR
ID - 2249820
AU - Hrbac, Tomas - Kopková, Alena - Siegl, František - Večeřa, Marek - Ručková, Michaela - Kazda, Tomáš - Jančálek, Radim - Hendrych, Michal - Hermanová, Markéta - Vybíhal, Václav - Fadrus, Pavel - Smrčka, Martin - Sokol, Filip - Kubeš, Václav - Lipina, Radim - Slabý, Ondřej - Křen, Leoš - Šána, Jiří
PY - 2022
TI - HLA-E and HLA-F Are Overexpressed in Glioblastoma and HLA-E Increased After Exposure to Ionizing Radiation
JF - CANCER GENOMICS & PROTEOMICS
VL - 19
IS - 2
SP - 151-162
EP - 151-162
PB - INT INST ANTICANCER RESEARCH
SN - 11096535
KW - Glioblastoma
KW - non-classical human leukocyte antigens
KW - HLA-E
KW - HLA-F
KW - ionizing radiation
KW - prognosis
UR - https://cgp.iiarjournals.org/content/19/2/151.long
N2 - Background/Aim: Glioblastoma (GBM) is one of the deadliest human cancers responding very poorly to therapy. Although the central nervous system has been traditionally considered an immunologically privileged site with an enhanced immune response, GBM appears to benefit from this immunosuppressive milieu. Immunomodulatory molecules play an important role in immune tumor-host interactions. Non-classical human leukocyte antigens (HLA) class Ib molecules HLA-E, HLA-F, and HLA-G have been previously described to be involved in protecting semi-allogeneic fetal allografts from the maternal immune response and in transplant tolerance as well as tumoral immune escape. Unfortunately, their role in GBM remains poorly understood. Our study, therefore, aimed to characterize the relationship between the expression of these molecules in GBM on the transcriptional level and clinico-pathological and molecular features of GBM as well as the effect of ionizing radiation. Materials and Methods: We performed the analysis of HLA-E, HLA-F, and HLA-G mRNA expression in 69 GBM tissue samples and 21 non-tumor brain tissue samples (controls) by reverse transcription polymerase chain reaction. Furthermore, two primary GBM cell cultures had been irradiated to identify the effect of ionizing radiation on the expression of non-classical HLA molecules. Results: Analyses revealed that both HLA-E and HLA-F are significantly up-regulated in GBM samples. Subsequent survival analysis showed a significant association between low expression of HLA-E and shorter survival of GBM patients. The dysregulated expression of both molecules was also observed between patients with methylated and unmethylated O-6-methylguanine-DNA methyltransferase (MGMT) promoter. Finally, we showed that ionizing radiation increased HLA-E expression level in GBM cells in vitro. Conclusion: HLA-E and HLA-F play an important role in GBM biology and could be used as diagnostic biomarkers, and in the case of HLA-E also as a prognostic biomarker.
ER -
HRBAC, Tomas, Alena KOPKOVÁ, František SIEGL, Marek VEČEŘA, Michaela RUČKOVÁ, Tomáš KAZDA, Radim JANČÁLEK, Michal HENDRYCH, Markéta HERMANOVÁ, Václav VYBÍHAL, Pavel FADRUS, Martin SMRČKA, Filip SOKOL, Václav KUBEŠ, Radim LIPINA, Ondřej SLABÝ, Leoš KŘEN and Jiří ŠÁNA. HLA-E and HLA-F Are Overexpressed in Glioblastoma and HLA-E Increased After Exposure to Ionizing Radiation. \textit{CANCER GENOMICS \&{} PROTEOMICS}. ATHENS: INT INST ANTICANCER RESEARCH, 2022, vol.~19, No~2, p.~151-162. ISSN~1109-6535. Available from: https://dx.doi.org/10.21873/cgp.20311.
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