Detailed Information on Publication Record
2022
HLA-E and HLA-F Are Overexpressed in Glioblastoma and HLA-E Increased After Exposure to Ionizing Radiation
HRBAC, Tomas, Alena KOPKOVÁ, František SIEGL, Marek VEČEŘA, Michaela RUČKOVÁ et. al.Basic information
Original name
HLA-E and HLA-F Are Overexpressed in Glioblastoma and HLA-E Increased After Exposure to Ionizing Radiation
Authors
HRBAC, Tomas (203 Czech Republic), Alena KOPKOVÁ (203 Czech Republic, belonging to the institution), František SIEGL (203 Czech Republic, belonging to the institution), Marek VEČEŘA (703 Slovakia, belonging to the institution), Michaela RUČKOVÁ (203 Czech Republic, belonging to the institution), Tomáš KAZDA (203 Czech Republic, belonging to the institution), Radim JANČÁLEK (203 Czech Republic, belonging to the institution), Michal HENDRYCH (203 Czech Republic, belonging to the institution), Markéta HERMANOVÁ (203 Czech Republic, belonging to the institution), Václav VYBÍHAL (203 Czech Republic), Pavel FADRUS (203 Czech Republic), Martin SMRČKA (203 Czech Republic), Filip SOKOL (203 Czech Republic), Václav KUBEŠ (203 Czech Republic), Radim LIPINA (203 Czech Republic), Ondřej SLABÝ (203 Czech Republic, belonging to the institution), Leoš KŘEN (203 Czech Republic, guarantor) and Jiří ŠÁNA (203 Czech Republic, belonging to the institution)
Edition
CANCER GENOMICS & PROTEOMICS, ATHENS, INT INST ANTICANCER RESEARCH, 2022, 1109-6535
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30204 Oncology
Country of publisher
Greece
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.500
RIV identification code
RIV/00216224:14740/22:00128290
Organization unit
Central European Institute of Technology
UT WoS
000789145900004
Keywords in English
Glioblastoma; non-classical human leukocyte antigens; HLA-E; HLA-F; ionizing radiation; prognosis
Tags
International impact, Reviewed
Změněno: 15/10/2024 14:42, Ing. Martina Blahová
Abstract
V originále
Background/Aim: Glioblastoma (GBM) is one of the deadliest human cancers responding very poorly to therapy. Although the central nervous system has been traditionally considered an immunologically privileged site with an enhanced immune response, GBM appears to benefit from this immunosuppressive milieu. Immunomodulatory molecules play an important role in immune tumor-host interactions. Non-classical human leukocyte antigens (HLA) class Ib molecules HLA-E, HLA-F, and HLA-G have been previously described to be involved in protecting semi-allogeneic fetal allografts from the maternal immune response and in transplant tolerance as well as tumoral immune escape. Unfortunately, their role in GBM remains poorly understood. Our study, therefore, aimed to characterize the relationship between the expression of these molecules in GBM on the transcriptional level and clinico-pathological and molecular features of GBM as well as the effect of ionizing radiation. Materials and Methods: We performed the analysis of HLA-E, HLA-F, and HLA-G mRNA expression in 69 GBM tissue samples and 21 non-tumor brain tissue samples (controls) by reverse transcription polymerase chain reaction. Furthermore, two primary GBM cell cultures had been irradiated to identify the effect of ionizing radiation on the expression of non-classical HLA molecules. Results: Analyses revealed that both HLA-E and HLA-F are significantly up-regulated in GBM samples. Subsequent survival analysis showed a significant association between low expression of HLA-E and shorter survival of GBM patients. The dysregulated expression of both molecules was also observed between patients with methylated and unmethylated O-6-methylguanine-DNA methyltransferase (MGMT) promoter. Finally, we showed that ionizing radiation increased HLA-E expression level in GBM cells in vitro. Conclusion: HLA-E and HLA-F play an important role in GBM biology and could be used as diagnostic biomarkers, and in the case of HLA-E also as a prognostic biomarker.
Links
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