Detailed Information on Publication Record
2022
Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis
VLADEJIC, Jovanka, Fen YANG, Eva Dvořák TOMAŠTÍKOVÁ, Jaroslav DOLEŽEL, Jan PALEČEK et. al.Basic information
Original name
Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis
Authors
VLADEJIC, Jovanka, Fen YANG, Eva Dvořák TOMAŠTÍKOVÁ, Jaroslav DOLEŽEL, Jan PALEČEK (203 Czech Republic, belonging to the institution) and Aleš PEČINKA (guarantor)
Edition
Frontiers in Plant Science, Lausanne, Frontiers Media S.A. 2022, 1664-462X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.600
RIV identification code
RIV/00216224:14310/22:00129401
Organization unit
Faculty of Science
UT WoS
000901916200001
Keywords in English
DNA damage repair; genome stability; BRCT domain; BRCT5 domain; homologous recombination; Arabidopsis
Tags
Tags
International impact, Reviewed
Změněno: 4/4/2023 15:25, Mgr. Marie Šípková, DiS.
Abstract
V originále
The integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identification and analysis of four uncharacterized Arabidopsis BRCT5 DOMAIN CONTAINING PROTEINs (BCPs). Proteins with the BRCT5 domain are frequently involved in the maintenance of genome stability across eukaryotes. The screening for sensitivity to induced DNA damage identified BCP1 as the most interesting candidate. We show that BCP1 loss of function mutants are hypersensitive to various types of DNA damage and accumulate an increased number of dead cells in root apical meristems upon DNA damage. Analysis of publicly available sog1 transcriptomic and SOG1 genome-wide DNA binding data revealed that BCP1 is inducible by gamma radiation and is a direct target of this key DNA damage signaling transcription factor. Importantly, bcp1 plants showed a reduced frequency of somatic homologous recombination in response to both endogenous and induced DNA damage. Altogether, we identified a novel plant-specific DNA repair factor that acts downstream of SOG1 in homology-based repair.
Links
GA20-05095S, research and development project |
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