Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in ...

@article{2250028,
   author = {Vladejic, Jovanka and Yang, Fen and Tomaštíková, Eva Dvořák and Doležel, Jaroslav and Paleček, Jan and Pečinka, Aleš},
   article_location = {Lausanne},
   article_number = {December},
   doi = {http://dx.doi.org/10.3389/fpls.2022.1023358},
   keywords = {DNA damage repair; genome stability; BRCT domain; BRCT5 domain; homologous recombination; Arabidopsis},
   language = {eng},
   issn = {1664-462X},
   journal = {Frontiers in Plant Science},
   title = {Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis},
   url = {https://doi.org/10.3389/fpls.2022.1023358},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2250028
AU  - Vladejic, Jovanka - Yang, Fen - Tomaštíková, Eva Dvořák - Doležel, Jaroslav - Paleček, Jan - Pečinka, Aleš
PY  - 2022
TI  - Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis
JF  - Frontiers in Plant Science
VL  - 13
IS  - December
SP  - 1-14
EP  - 1-14
PB  - Frontiers Media S.A.
SN  - 1664462X
KW  - DNA damage repair
KW  - genome stability
KW  - BRCT domain
KW  - BRCT5 domain
KW  - homologous recombination
KW  - Arabidopsis
UR  - https://doi.org/10.3389/fpls.2022.1023358
N2  - The integrity of plant genetic information is constantly challenged by various internal and external factors. Therefore, plants use a sophisticated molecular network to identify, signal and repair damaged DNA. Here, we report on the identification and analysis of four uncharacterized Arabidopsis BRCT5 DOMAIN CONTAINING PROTEINs (BCPs). Proteins with the BRCT5 domain are frequently involved in the maintenance of genome stability across eukaryotes. The screening for sensitivity to induced DNA damage identified BCP1 as the most interesting candidate. We show that BCP1 loss of function mutants are hypersensitive to various types of DNA damage and accumulate an increased number of dead cells in root apical meristems upon DNA damage. Analysis of publicly available sog1 transcriptomic and SOG1 genome-wide DNA binding data revealed that BCP1 is inducible by gamma radiation and is a direct target of this key DNA damage signaling transcription factor. Importantly, bcp1 plants showed a reduced frequency of somatic homologous recombination in response to both endogenous and induced DNA damage. Altogether, we identified a novel plant-specific DNA repair factor that acts downstream of SOG1 in homology-based repair.
ER  - 

VLADEJIC, Jovanka, Fen YANG, Eva Dvořák TOMAŠTÍKOVÁ, Jaroslav DOLEŽEL, Jan PALEČEK and Aleš PEČINKA. Analysis of BRCT5 domain-containing proteins reveals a new component of DNA damage repair in Arabidopsis. \textit{Frontiers in Plant Science}. Lausanne: Frontiers Media S.A., 2022, vol.~13, December, p.~1-14. ISSN~1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2022.1023358.