k 2022

DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL MILK NUTRITION FOR INFANTS BY HPLC

CRHA, Tomáš and Jiří PAZOUREK

Basic information

Original name

DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL MILK NUTRITION FOR INFANTS BY HPLC

Authors

CRHA, Tomáš (203 Czech Republic, belonging to the institution) and Jiří PAZOUREK (203 Czech Republic, belonging to the institution)

Edition

Student Scientific Conference MUNI Pharm, Doctoral Students 2022, 2022

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

30107 Medicinal chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14160/22:00128322

Organization unit

Faculty of Pharmacy

ISBN

978-80-280-0105-6

Keywords in English

HILIC; ELSD; lactose; carbohydrates

Tags

Změněno: 27/1/2023 13:25, Mgr. Tomáš Crha

Abstract

V originále

HPLC (high performance liquid chromatography) in the HILIC mode (hydrophilic interaction chromatography) is currently very often used in pharmacy for the separation and determination of polar analytes on polar stationary phases (in our case saccharides on the polyol stationary phase). However, since carbohydrates do not contain a suitable chromophore in their structure, a conventional UV detector is unusable for the determination, a refractometric detector would require isocratic elution, and therefore an evaporative light scattering detector (ELSD) can be used to advantage.

Links

MUNI/A/1236/2021, interní kód MU
Name: Stanovení cukerného profilu v mléku a speciální mléčné výživě pomocí HPLC
Investor: Masaryk University