J 2022

High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina

MORANDO, Nicolas, Eliška VRBOVÁ, Asunta MELGAR, Roberto Daniel RABINOVICH, David ŠMAJS et. al.

Basic information

Original name

High frequency of Nichols-like strains and increased levels of macrolide resistance in Treponema pallidum in clinical samples from Buenos Aires, Argentina

Authors

MORANDO, Nicolas, Eliška VRBOVÁ (203 Czech Republic, belonging to the institution), Asunta MELGAR, Roberto Daniel RABINOVICH, David ŠMAJS (203 Czech Republic, belonging to the institution) and Maria A PANDO (guarantor)

Edition

Nature Scientific Reports, Berlin, NATURE RESEARCH, 2022, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.600

RIV identification code

RIV/00216224:14110/22:00128324

Organization unit

Faculty of Medicine

DOI

UT WoS

000862059200008

Keywords in English

Nichols-like strains; Treponema pallidum; macrolide resistance

Tags

Tags

International impact, Reviewed
Změněno: 27/1/2023 12:28, Mgr. Tereza Miškechová

Abstract

V originále

Globally, 94% of Treponema pallidum subsp. pallidum (TPA) clinical strains belong to the SS14-like group and 6% to the Nichols-like group, with a prevalence of macrolide resistance of 90%. Our goal was to determine whether local TPA strain distribution and macrolide resistance frequency have changed significantly since our last report, which revealed that Buenos Aires had a high frequency of Nichols-like strains (27%) and low levels of macrolide resistance (14%). Swab samples from patients with suspected syphilis were collected during 2015-2019 and loci TP0136, TP0548, TP0705 were sequenced in order to perform multilocus sequence typing. Strains were classified as Nichols-like or SS14-like. The presence of macrolide resistance-associated mutations was determined by examination of the 23S rDNA gene sequence. Of 46 typeable samples, 37% were classified as Nichols-like and 63% as SS14-like. Macrolide resistance prevalence was 45.7%. Seven allelic profiles were found, five were SS14-like and two were Nichols-like. The frequency of Nichols-like strains increased between studies (26.8% vs. 37%, p = 0.36). A dramatic increase was found in the frequency of macrolide resistant strains between studies (14.3% vs. 45.7%, p = 0.005). Our results are in agreement with international trends and underscore the need to pursue further TPA molecular typing studies in South America.