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KOVACIK, Jozef, Lenka HUSAKOVA, Petr BABULA and Ildiko MATUSIKOVA. Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals. International Journal of Molecular Sciences. BASEL: MDPI, 2022, vol. 23, No 23, p. 1-13. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms232314736.

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TY  - JOUR
ID  - 2250085
AU  - Kovacik, Jozef - Husakova, Lenka - Babula, Petr - Matusikova, Ildiko
PY  - 2022
TI  - Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 23
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 14220067
KW  - antioxidant molecules
KW  - heavy metals
KW  - reactive oxygen species
KW  - soil pollution
UR  - https://www.mdpi.com/1422-0067/23/23/14736
N2  - Cadmium (Cd) or nickel (Ni) were applied as a foliar spray (1 mu M solution over one month) to mimic air pollution and to monitor metabolic responses and oxidative stress in the pteridophyte species. Exogenous metals did not affect the metal content of the soil and had relatively little effect on the essential elements in leaves or rhizomes. The amounts of Cd and Ni were similar in treated leaves (7.2 mu g Cd or 5.3 mu g Ni/g DW in mature leaves compared with 0.4 mu g Cd or 1.2 mu g Ni/g DW in the respective control leaves), but Ni was more abundant in rhizomes (56.6 mu g Ni or 3.4 mu g Cd/g DW), resulting in a higher Cd translocation and bioaccumulation factor. The theoretical calculation revealed that ca. 4% of Cd and 5.5% of Ni from the applied solution per plant/pot was absorbed. Excess Cd induced stronger ROS production followed by changes in SOD and CAT activities, whereas nitric oxide (NO) stimulation was less intense, as detected by confocal microscopy. The hadrocentric vascular bundles in the petioles also showed higher ROS and NO signals under metal excess. This may be a sign of increased ROS formation, and high correlations were observed. Proteins and amino acids were stimulated by Cd or Ni application in individual organs, whereas phenols and flavonols were almost unaffected. The data suggest that even low levels of exogenous metals induce an oxidative imbalance, although no visible damage is observed, and that the responses of ferns to metals are similar to those of seed plants or algae.
ER  -

Basic information
Original name	Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals
Authors	KOVACIK, Jozef (703 Slovakia, guarantor), Lenka HUSAKOVA (56 Belgium), Petr BABULA (203 Czech Republic, belonging to the institution) and Ildiko MATUSIKOVA (703 Slovakia).
Edition	International Journal of Molecular Sciences, BASEL, MDPI, 2022, 1422-0067.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10608 Biochemistry and molecular biology
Country of publisher	Switzerland
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
Impact factor	Impact factor: 5.600
RIV identification code	RIV/00216224:14110/22:00128332
Organization unit	Faculty of Medicine
Doi	http://dx.doi.org/10.3390/ijms232314736
UT WoS	000896247500001
Keywords in English	antioxidant molecules; heavy metals; reactive oxygen species; soil pollution
Tags	14110515, rivok
Tags	International impact, Reviewed
Changed by	Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 27/1/2023 14:01.

Abstract
Cadmium (Cd) or nickel (Ni) were applied as a foliar spray (1 mu M solution over one month) to mimic air pollution and to monitor metabolic responses and oxidative stress in the pteridophyte species. Exogenous metals did not affect the metal content of the soil and had relatively little effect on the essential elements in leaves or rhizomes. The amounts of Cd and Ni were similar in treated leaves (7.2 mu g Cd or 5.3 mu g Ni/g DW in mature leaves compared with 0.4 mu g Cd or 1.2 mu g Ni/g DW in the respective control leaves), but Ni was more abundant in rhizomes (56.6 mu g Ni or 3.4 mu g Cd/g DW), resulting in a higher Cd translocation and bioaccumulation factor. The theoretical calculation revealed that ca. 4% of Cd and 5.5% of Ni from the applied solution per plant/pot was absorbed. Excess Cd induced stronger ROS production followed by changes in SOD and CAT activities, whereas nitric oxide (NO) stimulation was less intense, as detected by confocal microscopy. The hadrocentric vascular bundles in the petioles also showed higher ROS and NO signals under metal excess. This may be a sign of increased ROS formation, and high correlations were observed. Proteins and amino acids were stimulated by Cd or Ni application in individual organs, whereas phenols and flavonols were almost unaffected. The data suggest that even low levels of exogenous metals induce an oxidative imbalance, although no visible damage is observed, and that the responses of ferns to metals are similar to those of seed plants or algae.

Abstract

Cadmium (Cd) or nickel (Ni) were applied as a foliar spray (1 mu M solution over one month) to mimic air pollution and to monitor metabolic responses and oxidative stress in the pteridophyte species. Exogenous metals did not affect the metal content of the soil and had relatively little effect on the essential elements in leaves or rhizomes. The amounts of Cd and Ni were similar in treated leaves (7.2 mu g Cd or 5.3 mu g Ni/g DW in mature leaves compared with 0.4 mu g Cd or 1.2 mu g Ni/g DW in the respective control leaves), but Ni was more abundant in rhizomes (56.6 mu g Ni or 3.4 mu g Cd/g DW), resulting in a higher Cd translocation and bioaccumulation factor. The theoretical calculation revealed that ca. 4% of Cd and 5.5% of Ni from the applied solution per plant/pot was absorbed. Excess Cd induced stronger ROS production followed by changes in SOD and CAT activities, whereas nitric oxide (NO) stimulation was less intense, as detected by confocal microscopy. The hadrocentric vascular bundles in the petioles also showed higher ROS and NO signals under metal excess. This may be a sign of increased ROS formation, and high correlations were observed. Proteins and amino acids were stimulated by Cd or Ni application in individual organs, whereas phenols and flavonols were almost unaffected. The data suggest that even low levels of exogenous metals induce an oxidative imbalance, although no visible damage is observed, and that the responses of ferns to metals are similar to those of seed plants or algae.