Metabolic and Oxidative Changes in the Fern Adiantum raddianum
upon Foliar Application of Metals

J 2022

Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals

KOVACIK, Jozef, Lenka HUSAKOVA, Petr BABULA and Ildiko MATUSIKOVA

Basic information

Original name

Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals

Authors

KOVACIK, Jozef (703 Slovakia, guarantor), Lenka HUSAKOVA (56 Belgium), Petr BABULA (203 Czech Republic, belonging to the institution) and Ildiko MATUSIKOVA (703 Slovakia)

Edition

International Journal of Molecular Sciences, BASEL, MDPI, 2022, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.600

RIV identification code

RIV/00216224:14110/22:00128332

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms232314736

UT WoS

000896247500001

Keywords in English

antioxidant molecules; heavy metals; reactive oxygen species; soil pollution

Abstract

V originále

Cadmium (Cd) or nickel (Ni) were applied as a foliar spray (1 mu M solution over one month) to mimic air pollution and to monitor metabolic responses and oxidative stress in the pteridophyte species. Exogenous metals did not affect the metal content of the soil and had relatively little effect on the essential elements in leaves or rhizomes. The amounts of Cd and Ni were similar in treated leaves (7.2 mu g Cd or 5.3 mu g Ni/g DW in mature leaves compared with 0.4 mu g Cd or 1.2 mu g Ni/g DW in the respective control leaves), but Ni was more abundant in rhizomes (56.6 mu g Ni or 3.4 mu g Cd/g DW), resulting in a higher Cd translocation and bioaccumulation factor. The theoretical calculation revealed that ca. 4% of Cd and 5.5% of Ni from the applied solution per plant/pot was absorbed. Excess Cd induced stronger ROS production followed by changes in SOD and CAT activities, whereas nitric oxide (NO) stimulation was less intense, as detected by confocal microscopy. The hadrocentric vascular bundles in the petioles also showed higher ROS and NO signals under metal excess. This may be a sign of increased ROS formation, and high correlations were observed. Proteins and amino acids were stimulated by Cd or Ni application in individual organs, whereas phenols and flavonols were almost unaffected. The data suggest that even low levels of exogenous metals induce an oxidative imbalance, although no visible damage is observed, and that the responses of ferns to metals are similar to those of seed plants or algae.

Citovat

KOVACIK, Jozef, Lenka HUSAKOVA, Petr BABULA and Ildiko MATUSIKOVA. Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals. International Journal of Molecular Sciences. BASEL: MDPI, 2022, vol. 23, No 23, p. 1-13. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms232314736.

@article{2250085,
   author = {Kovacik, Jozef and Husakova, Lenka and Babula, Petr and Matusikova, Ildiko},
   article_location = {BASEL},
   article_number = {23},
   doi = {http://dx.doi.org/10.3390/ijms232314736},
   keywords = {antioxidant molecules; heavy metals; reactive oxygen species; soil pollution},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals},
   url = {https://www.mdpi.com/1422-0067/23/23/14736},
   volume = {23},
   year = {2022}
}

TY  - JOUR
ID  - 2250085
AU  - Kovacik, Jozef - Husakova, Lenka - Babula, Petr - Matusikova, Ildiko
PY  - 2022
TI  - Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals
JF  - International Journal of Molecular Sciences
VL  - 23
IS  - 23
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 14220067
KW  - antioxidant molecules
KW  - heavy metals
KW  - reactive oxygen species
KW  - soil pollution
UR  - https://www.mdpi.com/1422-0067/23/23/14736
N2  - Cadmium (Cd) or nickel (Ni) were applied as a foliar spray (1 mu M solution over one month) to mimic air pollution and to monitor metabolic responses and oxidative stress in the pteridophyte species. Exogenous metals did not affect the metal content of the soil and had relatively little effect on the essential elements in leaves or rhizomes. The amounts of Cd and Ni were similar in treated leaves (7.2 mu g Cd or 5.3 mu g Ni/g DW in mature leaves compared with 0.4 mu g Cd or 1.2 mu g Ni/g DW in the respective control leaves), but Ni was more abundant in rhizomes (56.6 mu g Ni or 3.4 mu g Cd/g DW), resulting in a higher Cd translocation and bioaccumulation factor. The theoretical calculation revealed that ca. 4% of Cd and 5.5% of Ni from the applied solution per plant/pot was absorbed. Excess Cd induced stronger ROS production followed by changes in SOD and CAT activities, whereas nitric oxide (NO) stimulation was less intense, as detected by confocal microscopy. The hadrocentric vascular bundles in the petioles also showed higher ROS and NO signals under metal excess. This may be a sign of increased ROS formation, and high correlations were observed. Proteins and amino acids were stimulated by Cd or Ni application in individual organs, whereas phenols and flavonols were almost unaffected. The data suggest that even low levels of exogenous metals induce an oxidative imbalance, although no visible damage is observed, and that the responses of ferns to metals are similar to those of seed plants or algae.
ER  -

KOVACIK, Jozef, Lenka HUSAKOVA, Petr BABULA and Ildiko MATUSIKOVA. Metabolic and Oxidative Changes in the Fern Adiantum raddianum upon Foliar Application of Metals. \textit{International Journal of Molecular Sciences}. BASEL: MDPI, 2022, vol.~23, No~23, p.~1-13. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms232314736.

Detailed Information on Publication Record