CRHA, Tomáš and Jiří PAZOUREK. DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL INFANT’S FORMULAS BY HPLC. In 50th Conference SYNTHESIS AND ANALYSIS OF DRUGS. 2022. ISBN 978-80-280-0110-0.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL INFANT’S FORMULAS BY HPLC
Authors CRHA, Tomáš (203 Czech Republic, belonging to the institution) and Jiří PAZOUREK (203 Czech Republic, belonging to the institution).
Edition 50th Conference SYNTHESIS AND ANALYSIS OF DRUGS, 2022.
Other information
Original language English
Type of outcome Presentations at conferences
Field of Study 30107 Medicinal chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14160/22:00128333
Organization unit Faculty of Pharmacy
ISBN 978-80-280-0110-0
Keywords in English HILIC; ELSD; lactose; sugars; milk; infant formula
Tags rivok, ÚChL
Changed by Changed by: JUDr. Sabina Krejčiříková, učo 383857. Changed: 1/2/2023 11:22.
Abstract
HPLC (high performance liquid chromatography) in the HILIC mode (hydrophilic interaction chromatography) is currently very often used in pharmacy for the separation and determination of polar analytes on polar stationary phases (in our case saccharides on the polyol stationary phase). However, since carbohydrates do not contain a suitable chromophore in their structure, a conventional UV detector is unusable for the determination, a refractometric detector would require isocratic elution, and therefore an evaporative light scattering detector (ELSD) can be used to advantage. An HPLC-ELSD method was developed for milk sugar profiling. Lactose was determined and quantified in selected commercially available infant formula samples.
Links
MUNI/A/1236/2021, interní kód MUName: Stanovení cukerného profilu v mléku a speciální mléčné výživě pomocí HPLC
Investor: Masaryk University
PrintDisplayed: 18/8/2024 16:51