Detailed Information on Publication Record
2022
DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL INFANT’S FORMULAS BY HPLC
CRHA, Tomáš and Jiří PAZOUREKBasic information
Original name
DETERMINATION OF SUGAR PROFILE IN MILK AND SPECIAL INFANT’S FORMULAS BY HPLC
Authors
CRHA, Tomáš (203 Czech Republic, belonging to the institution) and Jiří PAZOUREK (203 Czech Republic, belonging to the institution)
Edition
50th Conference SYNTHESIS AND ANALYSIS OF DRUGS, 2022
Other information
Language
English
Type of outcome
Prezentace na konferencích
Field of Study
30107 Medicinal chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14160/22:00128333
Organization unit
Faculty of Pharmacy
ISBN
978-80-280-0110-0
Keywords in English
HILIC; ELSD; lactose; sugars; milk; infant formula
Změněno: 1/2/2023 11:22, JUDr. Sabina Krejčiříková
Abstract
V originále
HPLC (high performance liquid chromatography) in the HILIC mode (hydrophilic interaction chromatography) is currently very often used in pharmacy for the separation and determination of polar analytes on polar stationary phases (in our case saccharides on the polyol stationary phase). However, since carbohydrates do not contain a suitable chromophore in their structure, a conventional UV detector is unusable for the determination, a refractometric detector would require isocratic elution, and therefore an evaporative light scattering detector (ELSD) can be used to advantage. An HPLC-ELSD method was developed for milk sugar profiling. Lactose was determined and quantified in selected commercially available infant formula samples.
Links
| MUNI/A/1236/2021, interní kód MU |
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