FURMANCIKOVA, Petra, Zora STASTKOVA, Pavlina NAVRATILOVA, Ivana BEDNAROVA and Milada DVOŘÁČKOVÁ. Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic. Journal of Food and Nutrition Research. Bratislava: VUP FOOD RESEARCH INST, 2022, vol. 61, No 2, p. 192-197. ISSN 1336-8672.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic
Authors FURMANCIKOVA, Petra (203 Czech Republic, guarantor), Zora STASTKOVA (203 Czech Republic), Pavlina NAVRATILOVA (203 Czech Republic), Ivana BEDNAROVA (203 Czech Republic) and Milada DVOŘÁČKOVÁ (203 Czech Republic, belonging to the institution).
Edition Journal of Food and Nutrition Research, Bratislava, VUP FOOD RESEARCH INST, 2022, 1336-8672.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10606 Microbiology
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.100
RIV identification code RIV/00216224:14110/22:00128355
Organization unit Faculty of Medicine
UT WoS 000821448700009
Keywords in English Helicobacter pylori; nested polymerase chain reaction; food safety; raw milk
Tags 14110113, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 30/1/2023 12:44.
Abstract
Helicobacter pylori is currently found in more than a half of the human population and is the cause of serious gastrointestinal diseases such as gastritis, gastric ulcers or adenocarcinoma. H. pylori infection can be transmitted to humans through contaminated raw materials and food of animal origin. In this study, individual cows' milk samples (n = 77) and individual goats' milk samples (n = 52) from two farms in Moravia (Czech Republic) were analysed. The classical culture method and the nested polymerase chain reaction (nested PCR) method were used for detection of H. pylori. The culture method did not demonstrate the presence of H. pylori in any of the examined samples. Using the nested PCR method, 31 samples (40 %) of raw cows' milk and 30 samples (58 %) of raw goats' milk were positive for the presence of H. pylori. The study demonstrated that nested-PCR was highly sensitive for the detection of H. pylori in raw milk samples. The results of the study showed the presence of H. pylori DNA in a high percentage of both cows' and goats' raw milk samples, indicating a high possibility of inter-animal transmission within individual farms.
PrintDisplayed: 18/8/2024 05:26