L-Aspartate and L-Glutamine Inhibit Beta-Aminobutyric
Acid-Induced Resistance in Tomatoes

J 2022

L-Aspartate and L-Glutamine Inhibit Beta-Aminobutyric Acid-Induced Resistance in Tomatoes

JANOTÍK, Adam, Kateřina DADÁKOVÁ, Jan LOCHMAN and Martina ZAPLETALOVÁ

Basic information

Original name

L-Aspartate and L-Glutamine Inhibit Beta-Aminobutyric Acid-Induced Resistance in Tomatoes

Authors

JANOTÍK, Adam (703 Slovakia, belonging to the institution), Kateřina DADÁKOVÁ (203 Czech Republic, belonging to the institution), Jan LOCHMAN (203 Czech Republic, belonging to the institution) and Martina ZAPLETALOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plants, MDPI, 2022, 2223-7747

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.500

RIV identification code

RIV/00216224:14310/22:00128428

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3390/plants11212908

UT WoS

000881605300001

Keywords in English

beta-aminobutyric acid; BABA-induced resistance; Pseudomonas syringae; jasmonic acid; amino acids

Abstract

V originále

Plant diseases caused by pathogens lead to economic and agricultural losses, while plant resistance is defined by robustness and timing of defence response. Exposure to microbial-associated molecular patterns or specific chemical compounds can promote plants into a primed state with more robust defence responses. β-aminobutyric acid (BABA) is an endogenous stress metabolite that induces resistance, thereby protecting various plants’ diverse stresses by induction of non-canonical activity after binding into aspartyl-tRNA synthetase (AspRS). In this study, by integrating BABA-induced changes in selected metabolites and transcript data, we describe the molecular processes involved in BABA-induced resistance (BABA-IR) in tomatoes. BABA significantly restricted the growth of the pathogens P. syringae pv. tomato DC3000 and was related to the accumulation of transcripts for pathogenesis-related proteins and jasmonic acid signalling but not salicylic acid signalling in Arabidopsis. The resistance was considerably reduced by applying amino acids L-Asp and L-Gln when L-Gln prevents general amino acid inhibition in plants. Analysis of amino acid changes suggests that BABA-IR inhibition by L-Asp is due to its rapid metabolisation to L-Gln and not its competition with BABA for the aspartyl-tRNA synthetase (AspRS) binding site. Our results showed differences between the effect of BABA on tomatoes and other model plants. They highlighted the importance of comparative studies between plants of agronomic interest subjected to treatment with BABA.

Links

MUNI/A/1492/2021, interní kód MU

Name: Podpora biochemického výzkumu v roce 2022

Investor: Masaryk University

Citovat

JANOTÍK, Adam, Kateřina DADÁKOVÁ, Jan LOCHMAN and Martina ZAPLETALOVÁ. L-Aspartate and L-Glutamine Inhibit Beta-Aminobutyric Acid-Induced Resistance in Tomatoes. Plants. MDPI, 2022, vol. 11, No 21, p. 1-12. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants11212908.

@article{2251379,
   author = {Janotík, Adam and Dadáková, Kateřina and Lochman, Jan and Zapletalová, Martina},
   article_number = {21},
   doi = {http://dx.doi.org/10.3390/plants11212908},
   keywords = {beta-aminobutyric acid; BABA-induced resistance; Pseudomonas syringae; jasmonic acid; amino acids},
   language = {eng},
   issn = {2223-7747},
   journal = {Plants},
   title = {L-Aspartate and L-Glutamine Inhibit Beta-Aminobutyric Acid-Induced Resistance in Tomatoes},
   url = {https://www.mdpi.com/2223-7747/11/21/2908},
   volume = {11},
   year = {2022}
}

TY  - JOUR
ID  - 2251379
AU  - Janotík, Adam - Dadáková, Kateřina - Lochman, Jan - Zapletalová, Martina
PY  - 2022
TI  - L-Aspartate and L-Glutamine Inhibit Beta-Aminobutyric Acid-Induced Resistance in Tomatoes
JF  - Plants
VL  - 11
IS  - 21
SP  - 1-12
EP  - 1-12
PB  - MDPI
SN  - 22237747
KW  - beta-aminobutyric acid
KW  - BABA-induced resistance
KW  - Pseudomonas syringae
KW  - jasmonic acid
KW  - amino acids
UR  - https://www.mdpi.com/2223-7747/11/21/2908
N2  - Plant diseases caused by pathogens lead to economic and agricultural losses, while plant resistance is defined by robustness and timing of defence response. Exposure to microbial-associated molecular patterns or specific chemical compounds can promote plants into a primed state with more robust defence responses. β-aminobutyric acid (BABA) is an endogenous stress metabolite that induces resistance, thereby protecting various plants’ diverse stresses by induction of non-canonical activity after binding into aspartyl-tRNA synthetase (AspRS). In this study, by integrating BABA-induced changes in selected metabolites and transcript data, we describe the molecular processes involved in BABA-induced resistance (BABA-IR) in tomatoes. BABA significantly restricted the growth of the pathogens P. syringae pv. tomato DC3000 and was related to the accumulation of transcripts for pathogenesis-related proteins and jasmonic acid signalling but not salicylic acid signalling in Arabidopsis. The resistance was considerably reduced by applying amino acids L-Asp and L-Gln when L-Gln prevents general amino acid inhibition in plants. Analysis of amino acid changes suggests that BABA-IR inhibition by L-Asp is due to its rapid metabolisation to L-Gln and not its competition with BABA for the aspartyl-tRNA synthetase (AspRS) binding site. Our results showed differences between the effect of BABA on tomatoes and other model plants. They highlighted the importance of comparative studies between plants of agronomic interest subjected to treatment with BABA.
ER  -

JANOTÍK, Adam, Kateřina DADÁKOVÁ, Jan LOCHMAN and Martina ZAPLETALOVÁ. L-Aspartate and L-Glutamine Inhibit Beta-Aminobutyric Acid-Induced Resistance in Tomatoes. \textit{Plants}. MDPI, 2022, vol.~11, No~21, p.~1-12. ISSN~2223-7747. Available from: https://dx.doi.org/10.3390/plants11212908.

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