Influence of protein corona on the interaction of
glycogen-siRNA constructs with ex vivo human blood immune cells

J 2022

Influence of protein corona on the interaction of glycogen-siRNA constructs with ex vivo human blood immune cells

WOJNILOWICZ, Marcin, Petra LÁZNIČKOVÁ, Yi JU, Ching-Seng ANG, Federico TIDU et. al.

Basic information

Original name

Influence of protein corona on the interaction of glycogen-siRNA constructs with ex vivo human blood immune cells

Authors

WOJNILOWICZ, Marcin, Petra LÁZNIČKOVÁ (203 Czech Republic, belonging to the institution), Yi JU, Ching-Seng ANG, Federico TIDU (380 Italy), Kamila BENDICKOVA, Giancarlo FORTE (380 Italy), Magdalena PLEBANSKI, Frank CARUSO, Francesca CAVALIERI and Jan FRIČ (203 Czech Republic, guarantor)

Edition

BIOMATERIALS ADVANCES, AMSTERDAM, ELSEVIER, 2022, 2772-9508

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30404 Biomaterials

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14110/22:00128439

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.bioadv.2022.213083

UT WoS

000861397600003

Keywords in English

Glycogen nanoparticles; siRNA glycoplexes; Peripheral blood mononuclear cells; THP-1; Phagocytosis; Protein corona; Stochastic optical reconstruction microscopy

Abstract

V originále

Glycogen-nucleic acid constructs i.e., glycoplexes are emerging promising platforms for the alteration of gene expression and transcription. Understanding the interaction of glycoplexes with human blood components, such as serum proteins and peripheral blood mononuclear cells (PBMCs), is important to overcome immune cell activation and control biodistribution upon administration of the glycoplexes in vivo. Herein, we investigated the interactions of polyethylene glycol (PEG)ylated and non-PEGylated glycoplexes carrying siRNA molecules with PBMCs isolated from the blood of healthy donors. We found that both types of glycoplexes were non-toxic and were primarily phagocytosed by monocytes without triggering a pro-inflammatory interleukin 6 cytokine pro-duction. Furthermore, we investigated the role of the protein corona on controlling the internalization efficiency in immune cells - we found that the adsorption of serum proteins, in particular haptoglobin, alpha-1-antitrypsin and apolipoprotein A-II, onto the non-PEGylated glycoplexes, significantly reduced the uptake of the glycoplexes by PBMCs. Moreover, the non-PEGylated glycoplexes were efficient in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) knockdown in monocytic THP-1 cell line. This study provides an insight into the rational design of glycogen-based nanocarriers for the safe delivery of siRNA without eliciting unwanted immune cell activation and efficient siRNA activity upon its delivery.

Citovat

WOJNILOWICZ, Marcin, Petra LÁZNIČKOVÁ, Yi JU, Ching-Seng ANG, Federico TIDU, Kamila BENDICKOVA, Giancarlo FORTE, Magdalena PLEBANSKI, Frank CARUSO, Francesca CAVALIERI and Jan FRIČ. Influence of protein corona on the interaction of glycogen-siRNA constructs with ex vivo human blood immune cells. BIOMATERIALS ADVANCES. AMSTERDAM: ELSEVIER, 2022, vol. 140, September 2022, p. 1-13. ISSN 2772-9508. Available from: https://dx.doi.org/10.1016/j.bioadv.2022.213083.

@article{2251432,
   author = {Wojnilowicz, Marcin and Lázničková, Petra and Ju, Yi and Ang, ChingandSeng and Tidu, Federico and Bendickova, Kamila and Forte, Giancarlo and Plebanski, Magdalena and Caruso, Frank and Cavalieri, Francesca and Frič, Jan},
   article_location = {AMSTERDAM},
   article_number = {September 2022},
   doi = {http://dx.doi.org/10.1016/j.bioadv.2022.213083},
   keywords = {Glycogen nanoparticles; siRNA glycoplexes; Peripheral blood mononuclear cells; THP-1; Phagocytosis; Protein corona; Stochastic optical reconstruction microscopy},
   language = {eng},
   issn = {2772-9508},
   journal = {BIOMATERIALS ADVANCES},
   title = {Influence of protein corona on the interaction of glycogen-siRNA constructs with ex vivo human blood immune cells},
   url = {https://www.sciencedirect.com/science/article/pii/S2772950822003600?via%3Dihub},
   volume = {140},
   year = {2022}
}

TY  - JOUR
ID  - 2251432
AU  - Wojnilowicz, Marcin - Lázničková, Petra - Ju, Yi - Ang, Ching-Seng - Tidu, Federico - Bendickova, Kamila - Forte, Giancarlo - Plebanski, Magdalena - Caruso, Frank - Cavalieri, Francesca - Frič, Jan
PY  - 2022
TI  - Influence of protein corona on the interaction of glycogen-siRNA constructs with ex vivo human blood immune cells
JF  - BIOMATERIALS ADVANCES
VL  - 140
IS  - September 2022
SP  - 1-13
EP  - 1-13
PB  - ELSEVIER
SN  - 27729508
KW  - Glycogen nanoparticles
KW  - siRNA glycoplexes
KW  - Peripheral blood mononuclear cells
KW  - THP-1
KW  - Phagocytosis
KW  - Protein corona
KW  - Stochastic optical reconstruction microscopy
UR  - https://www.sciencedirect.com/science/article/pii/S2772950822003600?via%3Dihub
N2  - Glycogen-nucleic acid constructs i.e., glycoplexes are emerging promising platforms for the alteration of gene expression and transcription. Understanding the interaction of glycoplexes with human blood components, such as serum proteins and peripheral blood mononuclear cells (PBMCs), is important to overcome immune cell activation and control biodistribution upon administration of the glycoplexes in vivo. Herein, we investigated the interactions of polyethylene glycol (PEG)ylated and non-PEGylated glycoplexes carrying siRNA molecules with PBMCs isolated from the blood of healthy donors. We found that both types of glycoplexes were non-toxic and were primarily phagocytosed by monocytes without triggering a pro-inflammatory interleukin 6 cytokine pro-duction. Furthermore, we investigated the role of the protein corona on controlling the internalization efficiency in immune cells - we found that the adsorption of serum proteins, in particular haptoglobin, alpha-1-antitrypsin and apolipoprotein A-II, onto the non-PEGylated glycoplexes, significantly reduced the uptake of the glycoplexes by PBMCs. Moreover, the non-PEGylated glycoplexes were efficient in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) knockdown in monocytic THP-1 cell line. This study provides an insight into the rational design of glycogen-based nanocarriers for the safe delivery of siRNA without eliciting unwanted immune cell activation and efficient siRNA activity upon its delivery.
ER  -

WOJNILOWICZ, Marcin, Petra LÁZNIČKOVÁ, Yi JU, Ching-Seng ANG, Federico TIDU, Kamila BENDICKOVA, Giancarlo FORTE, Magdalena PLEBANSKI, Frank CARUSO, Francesca CAVALIERI and Jan FRIČ. Influence of protein corona on the interaction of glycogen-siRNA constructs with ex vivo human blood immune cells. \textit{BIOMATERIALS ADVANCES}. AMSTERDAM: ELSEVIER, 2022, vol.~140, September 2022, p.~1-13. ISSN~2772-9508. Available from: https://dx.doi.org/10.1016/j.bioadv.2022.213083.

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