| DOMANSKI, Michal, Emil DEDIC, Maria Escura PEREZ, Antoine CLERY, Sebastien CAMPAGNE, Anne-Christine ULDRY, Sophie BRAGA, Manfred HELLER, Julius RABL, Pavel AFANASYEV, Daniel BOEHRINGER, Jiří NOVÁČEK, Frederic T ALLAIN and Oliver MUEHLEMANN. 40S hnRNP particles are a novel class of nuclear biomolecular condensates. Nucleic acids research. Oxford: Oxford University Press, 2022, vol. 50, No 11, p. 6300-6312. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkac457. |
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@article{2251549,
author = {Domanski, Michal and Dedic, Emil and Perez, Maria Escura and Clery, Antoine and Campagne, Sebastien and Uldry, AnneandChristine and Braga, Sophie and Heller, Manfred and Rabl, Julius and Afanasyev, Pavel and Boehringer, Daniel and Nováček, Jiří and Allain, Frederic T and Muehlemann, Oliver},
article_location = {Oxford},
article_number = {11},
doi = {http://dx.doi.org/10.1093/nar/gkac457},
keywords = {RNA-BINDING PROTEINSRIBONUCLEOPROTEIN COMPLEX HNRNPPHASE-SEPARATIONMESSENGER-RNASTRUCTURAL ORGANIZATIONLABEL-FREEA1IDENTIFICATIONPURIFICATIONNUCLEOTIDES},
language = {eng},
issn = {0305-1048},
journal = {Nucleic acids research},
title = {40S hnRNP particles are a novel class of nuclear biomolecular condensates},
url = {https://academic.oup.com/nar/article/50/11/6300/6605309?login=true},
volume = {50},
year = {2022}
}
TY - JOUR
ID - 2251549
AU - Domanski, Michal - Dedic, Emil - Perez, Maria Escura - Clery, Antoine - Campagne, Sebastien - Uldry, Anne-Christine - Braga, Sophie - Heller, Manfred - Rabl, Julius - Afanasyev, Pavel - Boehringer, Daniel - Nováček, Jiří - Allain, Frederic T - Muehlemann, Oliver
PY - 2022
TI - 40S hnRNP particles are a novel class of nuclear biomolecular condensates
JF - Nucleic acids research
VL - 50
IS - 11
SP - 6300-6312
EP - 6300-6312
PB - Oxford University Press
SN - 03051048
KW - RNA-BINDING PROTEINSRIBONUCLEOPROTEIN COMPLEX HNRNPPHASE-SEPARATIONMESSENGER-RNASTRUCTURAL ORGANIZATIONLABEL-FREEA1IDENTIFICATIONPURIFICATIONNUCLEOTIDES
UR - https://academic.oup.com/nar/article/50/11/6300/6605309?login=true
N2 - Heterogenous nuclear ribonucleoproteins (hnRNPs) are abundant proteins implicated in various steps of RNA processing that assemble on nuclear RNA into larger complexes termed 40S hnRNP particles. Despite their initial discovery 55 years ago, our understanding of these intriguing macromolecular assemblies remains limited. Here, we report the biochemical purification of native 40S hnRNP particles and the determination of their complete protein composition by label-free quantitative mass spectrometry, identifying A-group and C-group hnRNPs as the major protein constituents. Isolated 40S hnRNP particles dissociate upon RNA digestion and can be reconstituted in vitro on defined RNAs in the presence of the individual protein components, demonstrating a scaffolding role for RNA in nucleating particle formation. Finally, we revealed their nanometer scale, condensate-like nature, promoted by intrinsically disordered regions of A-group hnRNPs. Collectively, we identify nuclear 40S hnRNP particles as novel dynamic biomolecular condensates.
ER -
DOMANSKI, Michal, Emil DEDIC, Maria Escura PEREZ, Antoine CLERY, Sebastien CAMPAGNE, Anne-Christine ULDRY, Sophie BRAGA, Manfred HELLER, Julius RABL, Pavel AFANASYEV, Daniel BOEHRINGER, Jiří NOVÁČEK, Frederic T ALLAIN and Oliver MUEHLEMANN. 40S hnRNP particles are a novel class of nuclear biomolecular condensates. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2022, vol.~50, No~11, p.~6300-6312. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkac457.
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