Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions
SIMONOVA, Anna, Veronika ROMANSKA, Barbora BENONI, Karel ŠKUBNÍK, Lenka ŠMERDOVÁ, Michaela PROCHÁZKOVÁ, Kristina SPUSTOVA, Ondrej MORAVCIK, Lenka GAHUROVA, Jan PACES, Pavel PLEVKA and Hana CAHOVA. Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions. Chembiochem. WEINHEIM: WILEY-VCH, 2022, vol. 23, No 17, p. 202200281-202200289. ISSN 1439-4227. Available from: https://dx.doi.org/10.1002/cbic.202200281. |
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Basic information | |
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Original name | Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions |
Authors | SIMONOVA, Anna, Veronika ROMANSKA, Barbora BENONI, Karel ŠKUBNÍK (203 Czech Republic, belonging to the institution), Lenka ŠMERDOVÁ (203 Czech Republic, belonging to the institution), Michaela PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Kristina SPUSTOVA, Ondrej MORAVCIK, Lenka GAHUROVA, Jan PACES, Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution) and Hana CAHOVA. |
Edition | Chembiochem, WEINHEIM, WILEY-VCH, 2022, 1439-4227. |
Other information | |
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Original language | English |
Type of outcome | Article in a journal |
Field of Study | 10608 Biochemistry and molecular biology |
Country of publisher | Germany |
Confidentiality degree | is not subject to a state or trade secret |
WWW | URL |
Impact factor | Impact factor: 3.200 |
RIV identification code | RIV/00216224:14740/22:00128475 |
Organization unit | Central European Institute of Technology |
Doi | http://dx.doi.org/10.1002/cbic.202200281 |
UT WoS | 000828265300001 |
Keywords in English | human rhinovirus type 2; human Echovirus 18; LC-MS; Picornavirales; RNA methylation; tRNA fragments |
Tags | rivok |
Tags | International impact, Reviewed |
Changed by | Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 1/2/2023 12:24. |
Abstract |
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The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles; thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC-MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N-6-methyladenosine and 2'-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m(1)A) and 5-methylcytidine (m(5)C). Mapping of m(1)A and m(5)C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3' and 5' fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses. |
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LM2018131, research and development project | Name: Česká národní infrastruktura pro biologická data (Acronym: ELIXIR-CZ) |
Investor: Ministry of Education, Youth and Sports of the CR, Czech National Infrastructure for Biological Data |
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