Genic and chromosomal components of Prdm9-driven hybrid male
sterility in mice (Mus musculus)

J 2022

Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)

VALISKOVA, Barbora, Sona GREGOROVA, Diana LUSTYK, Petr ŠIMEČEK, Petr JANSA et. al.

Basic information

Original name

Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)

Authors

VALISKOVA, Barbora, Sona GREGOROVA, Diana LUSTYK, Petr ŠIMEČEK (203 Czech Republic, guarantor, belonging to the institution), Petr JANSA and Jiri FOREJT

Edition

Genetics, BETHESDA (USA), GENETICS SOCIETY AMERICA, 2022, 0016-6731

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.300

RIV identification code

RIV/00216224:14740/22:00128478

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/genetics/iyac116

UT WoS

000838737000001

Keywords in English

spermatogenesis; meiosis; homologous synapsis; SYCP3; HORMAD2; synaptonemal complex

Abstract

V originále

Hybrid sterility contributes to speciation by preventing gene flow between related taxa. Prdm9, the first and only hybrid male sterility gene known in vertebrates, predetermines the sites of recombination between homologous chromosomes and their synapsis in early meiotic prophase. The asymmetric binding of PRDM9 to heterosubspecific homologs of Mus musculus musculus x Mus musculus domesticus F1 hybrids and increase of PRDM9-independent DNA double-strand break hotspots results indificult- to- repair double-strand breaks, incomplete synapsis of homologous chromosomes, and meiotic arrest at the first meiotic prophase. Here, we show that Prdm9 behaves as a major hybrid male sterility gene in mice outside the Mus musculus musculus x Mus musculus domesticus F1 hybrids, in the genomes composed of Mus musculus castaneus and Mus musculus musculus chromosomes segregating on the Mus musculus domesticus background. The Prdm9(cst/dom2) (castaneus/domesticus) allelic combination secures meiotic synapsis, testes weight, and sperm count within physiological limits, while the Prdm9(msc1/dom2) (musculus/domesticus) males show a range of fertility impairment. Out of 5 quantitative trait loci contributing to the Prdm9(msc1/dom2)-related infertility, 4 control either meiotic synapsis or fertility phenotypes and 1 controls both, synapsis, and fertility. Whole-genome genotyping of individual chromosomes showed preferential involvement of nonrecombinant musculus chromosomes in asynapsis in accordance with the chromosomal character of hybrid male sterility. Moreover, we show that the overall asynapsis rate can be estimated solely from the genotype of individual males by scoring the effect of nonrecombinant musculus chromosomes. Prdm9-controlled hybrid male sterility represents an example of genetic architecture of hybrid male sterility consisting of genic and chromosomal components.

Links

90267, large research infrastructures

Name: NCMG III

Citovat

VALISKOVA, Barbora, Sona GREGOROVA, Diana LUSTYK, Petr ŠIMEČEK, Petr JANSA and Jiri FOREJT. Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus). Genetics. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2022, vol. 222, No 1, p. 1-14. ISSN 0016-6731. Available from: https://dx.doi.org/10.1093/genetics/iyac116.

@article{2251936,
   author = {Valiskova, Barbora and Gregorova, Sona and Lustyk, Diana and Šimeček, Petr and Jansa, Petr and Forejt, Jiri},
   article_location = {BETHESDA (USA)},
   article_number = {1},
   doi = {http://dx.doi.org/10.1093/genetics/iyac116},
   keywords = {spermatogenesis; meiosis; homologous synapsis; SYCP3; HORMAD2; synaptonemal complex},
   language = {eng},
   issn = {0016-6731},
   journal = {Genetics},
   title = {Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)},
   url = {https://academic.oup.com/genetics/article/222/1/iyac116/6655690?login=true},
   volume = {222},
   year = {2022}
}

TY  - JOUR
ID  - 2251936
AU  - Valiskova, Barbora - Gregorova, Sona - Lustyk, Diana - Šimeček, Petr - Jansa, Petr - Forejt, Jiri
PY  - 2022
TI  - Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus)
JF  - Genetics
VL  - 222
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - GENETICS SOCIETY AMERICA
SN  - 00166731
KW  - spermatogenesis
KW  - meiosis
KW  - homologous synapsis
KW  - SYCP3
KW  - HORMAD2
KW  - synaptonemal complex
UR  - https://academic.oup.com/genetics/article/222/1/iyac116/6655690?login=true
N2  - Hybrid sterility contributes to speciation by preventing gene flow between related taxa. Prdm9, the first and only hybrid male sterility gene known in vertebrates, predetermines the sites of recombination between homologous chromosomes and their synapsis in early meiotic prophase. The asymmetric binding of PRDM9 to heterosubspecific homologs of Mus musculus musculus x Mus musculus domesticus F1 hybrids and increase of PRDM9-independent DNA double-strand break hotspots results indificult- to- repair double-strand breaks, incomplete synapsis of homologous chromosomes, and meiotic arrest at the first meiotic prophase. Here, we show that Prdm9 behaves as a major hybrid male sterility gene in mice outside the Mus musculus musculus x Mus musculus domesticus F1 hybrids, in the genomes composed of Mus musculus castaneus and Mus musculus musculus chromosomes segregating on the Mus musculus domesticus background. The Prdm9(cst/dom2) (castaneus/domesticus) allelic combination secures meiotic synapsis, testes weight, and sperm count within physiological limits, while the Prdm9(msc1/dom2) (musculus/domesticus) males show a range of fertility impairment. Out of 5 quantitative trait loci contributing to the Prdm9(msc1/dom2)-related infertility, 4 control either meiotic synapsis or fertility phenotypes and 1 controls both, synapsis, and fertility. Whole-genome genotyping of individual chromosomes showed preferential involvement of nonrecombinant musculus chromosomes in asynapsis in accordance with the chromosomal character of hybrid male sterility. Moreover, we show that the overall asynapsis rate can be estimated solely from the genotype of individual males by scoring the effect of nonrecombinant musculus chromosomes. Prdm9-controlled hybrid male sterility represents an example of genetic architecture of hybrid male sterility consisting of genic and chromosomal components.
ER  -

VALISKOVA, Barbora, Sona GREGOROVA, Diana LUSTYK, Petr ŠIMEČEK, Petr JANSA and Jiri FOREJT. Genic and chromosomal components of Prdm9-driven hybrid male sterility in mice (Mus musculus). \textit{Genetics}. BETHESDA (USA): GENETICS SOCIETY AMERICA, 2022, vol.~222, No~1, p.~1-14. ISSN~0016-6731. Available from: https://dx.doi.org/10.1093/genetics/iyac116.

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