Characterization of insulin crystalline form in isolated
beta-cell secretory granules

J 2022

Characterization of insulin crystalline form in isolated beta-cell secretory granules

ASAI, Seiya, Jana MORAVCOVA, Lenka ZAKOVA, Irena SELICHAROVA, Romana HADRAVOVA et. al.

Basic information

Original name

Characterization of insulin crystalline form in isolated beta-cell secretory granules

Authors

ASAI, Seiya, Jana MORAVCOVA, Lenka ZAKOVA, Irena SELICHAROVA, Romana HADRAVOVA, Andrzej Marek BRZOZOWSKI, Jiří NOVÁČEK (203 Czech Republic, guarantor, belonging to the institution) and Jiri JIRACEK

Edition

Open Biology, 2022, 2046-2441

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.800

RIV identification code

RIV/00216224:14740/22:00128509

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1098/rsob.220322

UT WoS

000900974200003

Keywords in English

crystallization in vivo; peptide hormone; subcellular vesicle; electron microscopy; secretory granules; insulin secretion

Abstract

V originále

Insulin is stored in vivo inside the pancreatic beta-cell insulin secretory granules. In vitro studies have led to an assumption that high insulin and Zn2+ concentrations inside the pancreatic beta-cell insulin secretory granules should promote insulin crystalline state in the form of Zn2+-stabilized hexamers. Electron microscopic images of thin sections of the pancreatic beta-cells often show a dense, regular pattern core, suggesting the presence of insulin crystals. However, the structural features of the storage forms of insulin in native preparations of secretory granules are unknown, because of their small size, fragile character and difficult handling. We isolated and investigated the secretory granules from MIN6 cells under near-native conditions, using cryo-electron microscopic (Cryo-EM) techniques. The analysis of these data from multiple intra-granular crystals revealed two different rhomboidal crystal lattices. The minor lattice has unit cell parameters (a similar or equal to b similar or equal to 84.0 angstrom, c similar or equal to 35.2 angstrom), similar to in vitro crystallized human 4Zn(2+)-insulin hexamer, whereas the largely prevalent unit cell has more than double c-axis (a similar or equal to b similar or equal to c similar or equal to 96.5 angstrom) that probably corresponds to two or three insulin hexamers in the asymmetric unit. Our experimental data show that insulin can be present in pancreatic MIN6 cell granules in a microcrystalline form, probably consisting of 4Zn(2+)-hexamers of this hormone.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

ASAI, Seiya, Jana MORAVCOVA, Lenka ZAKOVA, Irena SELICHAROVA, Romana HADRAVOVA, Andrzej Marek BRZOZOWSKI, Jiří NOVÁČEK and Jiri JIRACEK. Characterization of insulin crystalline form in isolated beta-cell secretory granules. Open Biology. 2022, vol. 12, No 12, p. 220322-220331. ISSN 2046-2441. Available from: https://dx.doi.org/10.1098/rsob.220322.

@article{2252276,
   author = {Asai, Seiya and Moravcova, Jana and Zakova, Lenka and Selicharova, Irena and Hadravova, Romana and Brzozowski, Andrzej Marek and Nováček, Jiří and Jiracek, Jiri},
   article_number = {12},
   doi = {http://dx.doi.org/10.1098/rsob.220322},
   keywords = {crystallization in vivo; peptide hormone; subcellular vesicle; electron microscopy; secretory granules; insulin secretion},
   language = {eng},
   issn = {2046-2441},
   journal = {Open Biology},
   title = {Characterization of insulin crystalline form in isolated beta-cell secretory granules},
   url = {https://royalsocietypublishing.org/doi/10.1098/rsob.220322},
   volume = {12},
   year = {2022}
}

TY  - JOUR
ID  - 2252276
AU  - Asai, Seiya - Moravcova, Jana - Zakova, Lenka - Selicharova, Irena - Hadravova, Romana - Brzozowski, Andrzej Marek - Nováček, Jiří - Jiracek, Jiri
PY  - 2022
TI  - Characterization of insulin crystalline form in isolated beta-cell secretory granules
JF  - Open Biology
VL  - 12
IS  - 12
SP  - 220322
EP  - 220322
SN  - 20462441
KW  - crystallization in vivo
KW  - peptide hormone
KW  - subcellular vesicle
KW  - electron microscopy
KW  - secretory granules
KW  - insulin secretion
UR  - https://royalsocietypublishing.org/doi/10.1098/rsob.220322
N2  - Insulin is stored in vivo inside the pancreatic beta-cell insulin secretory granules. In vitro studies have led to an assumption that high insulin and Zn2+ concentrations inside the pancreatic beta-cell insulin secretory granules should promote insulin crystalline state in the form of Zn2+-stabilized hexamers. Electron microscopic images of thin sections of the pancreatic beta-cells often show a dense, regular pattern core, suggesting the presence of insulin crystals. However, the structural features of the storage forms of insulin in native preparations of secretory granules are unknown, because of their small size, fragile character and difficult handling. We isolated and investigated the secretory granules from MIN6 cells under near-native conditions, using cryo-electron microscopic (Cryo-EM) techniques. The analysis of these data from multiple intra-granular crystals revealed two different rhomboidal crystal lattices. The minor lattice has unit cell parameters (a similar or equal to b similar or equal to 84.0 angstrom, c similar or equal to 35.2 angstrom), similar to in vitro crystallized human 4Zn(2+)-insulin hexamer, whereas the largely prevalent unit cell has more than double c-axis (a similar or equal to b similar or equal to c similar or equal to 96.5 angstrom) that probably corresponds to two or three insulin hexamers in the asymmetric unit. Our experimental data show that insulin can be present in pancreatic MIN6 cell granules in a microcrystalline form, probably consisting of 4Zn(2+)-hexamers of this hormone.
ER  -

ASAI, Seiya, Jana MORAVCOVA, Lenka ZAKOVA, Irena SELICHAROVA, Romana HADRAVOVA, Andrzej Marek BRZOZOWSKI, Jiří NOVÁČEK and Jiri JIRACEK. Characterization of insulin crystalline form in isolated beta-cell secretory granules. \textit{Open Biology}. 2022, vol.~12, No~12, p.~220322-220331. ISSN~2046-2441. Available from: https://dx.doi.org/10.1098/rsob.220322.

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