Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB

JOURAVLEVA, Karina, Dmitrij GOLOVENKO, Gabriel DEMO, Robert C DUTCHER, Traci M Tanaka HALL, Phillip D ZAMORE and Andrei A KOROSTELEV. Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB. Molecular Cell. CAMBRIDGE: CELL PRESS, 2022, vol. 82, No 21, p. "4049"-"+", 22 pp. ISSN 1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2022.09.002.

Basic information

• Original name: Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB
• Authors: JOURAVLEVA, Karina, Dmitrij GOLOVENKO, Gabriel DEMO (703 Slovakia, guarantor, belonging to the institution), Robert C DUTCHER, Traci M Tanaka HALL, Phillip D ZAMORE and Andrei A KOROSTELEV.
• Edition: Molecular Cell, CAMBRIDGE, CELL PRESS, 2022, 1097-2765.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 16.000
• RIV identification code: RIV/00216224:14740/22:00128512
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.molcel.2022.09.002
• UT WoS: 000898565300010
• Keywords in English: COLI RIBONUCLEASE-IIIRNA-BINDINGCRYSTAL-STRUCTUREDOMAIN REVEALSTRBP COMPLEXRECOGNITIONMIRNASINTERFERENCEPREDICTIONMECHANISM
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) pro-teins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner Loqs-PB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicer-1???Loqs-PB heterodimer. The Dicer-1 dsRBD and three Loqs-PB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicer-1???Loqs-PB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release. Keywords

Links

• LL2008, research and development project: Name: Komunikace mezi transkripcí a translací (Acronym: CROSSBETT)

Investor: Ministry of Education, Youth and Sports of the CR, Crosstalk between transcription and translation