Structural basis of microRNA biogenesis by Dicer-1 and its
partner protein Loqs-PB

J 2022

Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB

JOURAVLEVA, Karina, Dmitrij GOLOVENKO, Gabriel DEMO, Robert C DUTCHER, Traci M Tanaka HALL et. al.

Basic information

Original name

Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB

Authors

JOURAVLEVA, Karina, Dmitrij GOLOVENKO, Gabriel DEMO (703 Slovakia, guarantor, belonging to the institution), Robert C DUTCHER, Traci M Tanaka HALL, Phillip D ZAMORE and Andrei A KOROSTELEV

Edition

Molecular Cell, CAMBRIDGE, CELL PRESS, 2022, 1097-2765

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 16.000

RIV identification code

RIV/00216224:14740/22:00128512

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.molcel.2022.09.002

UT WoS

000898565300010

Keywords in English

COLI RIBONUCLEASE-IIIRNA-BINDINGCRYSTAL-STRUCTUREDOMAIN REVEALSTRBP COMPLEXRECOGNITIONMIRNASINTERFERENCEPREDICTIONMECHANISM

Abstract

V originále

In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) pro-teins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner Loqs-PB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicer-1???Loqs-PB heterodimer. The Dicer-1 dsRBD and three Loqs-PB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicer-1???Loqs-PB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release. Keywords

Links

LL2008, research and development project

Name: Komunikace mezi transkripcí a translací (Acronym: CROSSBETT)

Investor: Ministry of Education, Youth and Sports of the CR, Crosstalk between transcription and translation

Citovat

JOURAVLEVA, Karina, Dmitrij GOLOVENKO, Gabriel DEMO, Robert C DUTCHER, Traci M Tanaka HALL, Phillip D ZAMORE and Andrei A KOROSTELEV. Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB. Molecular Cell. CAMBRIDGE: CELL PRESS, 2022, vol. 82, No 21, p. "4049"-"+", 22 pp. ISSN 1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2022.09.002.

@article{2252339,
   author = {Jouravleva, Karina and Golovenko, Dmitrij and Demo, Gabriel and Dutcher, Robert C and Hall, Traci M Tanaka and Zamore, Phillip D and Korostelev, Andrei A},
   article_location = {CAMBRIDGE},
   article_number = {21},
   doi = {http://dx.doi.org/10.1016/j.molcel.2022.09.002},
   keywords = {COLI RIBONUCLEASE-IIIRNA-BINDINGCRYSTAL-STRUCTUREDOMAIN REVEALSTRBP COMPLEXRECOGNITIONMIRNASINTERFERENCEPREDICTIONMECHANISM},
   language = {eng},
   issn = {1097-2765},
   journal = {Molecular Cell},
   title = {Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB},
   url = {https://www.sciencedirect.com/science/article/pii/S109727652200853X?via%3Dihub},
   volume = {82},
   year = {2022}
}

TY  - JOUR
ID  - 2252339
AU  - Jouravleva, Karina - Golovenko, Dmitrij - Demo, Gabriel - Dutcher, Robert C - Hall, Traci M Tanaka - Zamore, Phillip D - Korostelev, Andrei A
PY  - 2022
TI  - Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB
JF  - Molecular Cell
VL  - 82
IS  - 21
SP  - "4049"-"+"
EP  - "4049"-"+"
PB  - CELL PRESS
SN  - 10972765
KW  - COLI RIBONUCLEASE-IIIRNA-BINDINGCRYSTAL-STRUCTUREDOMAIN REVEALSTRBP COMPLEXRECOGNITIONMIRNASINTERFERENCEPREDICTIONMECHANISM
UR  - https://www.sciencedirect.com/science/article/pii/S109727652200853X?via%3Dihub
N2  - In animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding domain (dsRBD) pro-teins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 that show how Dicer-1 and its partner Loqs-PB cooperate (1) before binding pre-miRNA, (2) after binding and in a catalytically competent state, (3) after nicking one arm of the pre-miRNA, and (4) following complete dicing and initial product release. Our reconstructions suggest that pre-miRNA binds a rare, open conformation of the Dicer-1???Loqs-PB heterodimer. The Dicer-1 dsRBD and three Loqs-PB dsRBDs form a tight belt around the pre-miRNA, distorting the RNA helix to place the scissile phosphodiester bonds in the RNase III active sites. Pre-miRNA cleavage shifts the dsRBDs and partially closes Dicer-1, which may promote product release. Our data suggest a model for how the Dicer-1???Loqs-PB complex affects a complete cycle of pre-miRNA recognition, stepwise endonuclease cleavage, and product release. Keywords
ER  -

JOURAVLEVA, Karina, Dmitrij GOLOVENKO, Gabriel DEMO, Robert C DUTCHER, Traci M Tanaka HALL, Phillip D ZAMORE and Andrei A KOROSTELEV. Structural basis of microRNA biogenesis by Dicer-1 and its partner protein Loqs-PB. \textit{Molecular Cell}. CAMBRIDGE: CELL PRESS, 2022, vol.~82, No~21, p.~''4049''-''+'', 22 pp. ISSN~1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2022.09.002.

Detailed Information on Publication Record