SERS-Tags: Selective Immobilization and Detection of Bacteria
by Strain-Specific Antibodies and Surface-Enhanced Raman
Scattering

J 2023

SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering

BENEŠOVÁ, M., S. BERNATOVÁ, F. MIKA, Z. POKORNÁ, J. JEŽEK et. al.

Basic information

Original name

SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering

Authors

BENEŠOVÁ, M. (203 Czech Republic), S. BERNATOVÁ (203 Czech Republic), F. MIKA (203 Czech Republic), Z. POKORNÁ (203 Czech Republic), J. JEŽEK (203 Czech Republic), M. ŠILER (203 Czech Republic), O. SAMEK (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Katarína REBROŠOVÁ (703 Slovakia, belonging to the institution), P. ZEMÁNEK (203 Czech Republic) and Z. PILÁT (203 Czech Republic, guarantor)

Edition

Biosensors, Basel, MDPI, 2023, 2079-6374

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.400 in 2022

RIV identification code

RIV/00216224:14110/23:00134625

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/bios13020182

UT WoS

000938961100001

Keywords in English

SERS-tag; Escherichia coli; sandwich immunoassay; single-cell detection

Abstract

V originále

Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen Escherichia coli, and we demonstrate the selectivity of our method using two other bacterial strains, Staphylococcus aureus and Serratia marcescens, as negative controls. We believe that the described approach has a potential to inspire the development of novel medical diagnostic tools for rapid identification of bacterial pathogens.

Links

NU21-05-00341, research and development project

Name: Pokročilé metody detekce patogenních mikroorganizmů a hodnocení účinku antimikrobiálních látek Ramanovou spektroskopií a dalšími metodami na úrovni molekul v optofluidních systémech

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

BENEŠOVÁ, M., S. BERNATOVÁ, F. MIKA, Z. POKORNÁ, J. JEŽEK, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, Katarína REBROŠOVÁ, P. ZEMÁNEK and Z. PILÁT. SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering. Biosensors. Basel: MDPI, 2023, vol. 13, No 2, p. 1-14. ISSN 2079-6374. Available from: https://dx.doi.org/10.3390/bios13020182.

@article{2252384,
   author = {Benešová, M. and Bernatová, S. and Mika, F. and Pokorná, Z. and Ježek, J. and Šiler, M. and Samek, O. and Růžička, Filip and Rebrošová, Katarína and Zemánek, P. and Pilát, Z.},
   article_location = {Basel},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/bios13020182},
   keywords = {SERS-tag; Escherichia coli; sandwich immunoassay; single-cell detection},
   language = {eng},
   issn = {2079-6374},
   journal = {Biosensors},
   title = {SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering},
   url = {https://www.mdpi.com/2079-6374/13/2/182},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2252384
AU  - Benešová, M. - Bernatová, S. - Mika, F. - Pokorná, Z. - Ježek, J. - Šiler, M. - Samek, O. - Růžička, Filip - Rebrošová, Katarína - Zemánek, P. - Pilát, Z.
PY  - 2023
TI  - SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering
JF  - Biosensors
VL  - 13
IS  - 2
SP  - 1-14
EP  - 1-14
PB  - MDPI
SN  - 20796374
KW  - SERS-tag
KW  - Escherichia coli
KW  - sandwich immunoassay
KW  - single-cell detection
UR  - https://www.mdpi.com/2079-6374/13/2/182
N2  - Efficient separation and sensitive identification of pathogenic bacterial strains is essential for a prosperous modern society, with direct applications in medical diagnostics, drug discovery, biodefense, and food safety. We developed a fast and reliable method for antibody-based selective immobilization of bacteria from suspension onto a gold-plated glass surface, followed by detection using strain-specific antibodies linked to gold nanoparticles decorated with a reporter molecule. The reporter molecules are subsequently detected by surface-enhanced Raman spectroscopy (SERS). Such a multi-functionalized nanoparticle is called a SERS-tag. The presented procedure uses widely accessible and cheap materials for manufacturing and functionalization of the nanoparticles and the immobilization surfaces. Here, we exemplify the use of the produced SERS-tags for sensitive single-cell detection of opportunistic pathogen Escherichia coli, and we demonstrate the selectivity of our method using two other bacterial strains, Staphylococcus aureus and Serratia marcescens, as negative controls. We believe that the described approach has a potential to inspire the development of novel medical diagnostic tools for rapid identification of bacterial pathogens.
ER  -

BENEŠOVÁ, M., S. BERNATOVÁ, F. MIKA, Z. POKORNÁ, J. JEŽEK, M. ŠILER, O. SAMEK, Filip RŮŽIČKA, Katarína REBROŠOVÁ, P. ZEMÁNEK and Z. PILÁT. SERS-Tags: Selective Immobilization and Detection of Bacteria by Strain-Specific Antibodies and Surface-Enhanced Raman Scattering. \textit{Biosensors}. Basel: MDPI, 2023, vol.~13, No~2, p.~1-14. ISSN~2079-6374. Available from: https://dx.doi.org/10.3390/bios13020182.

Detailed Information on Publication Record