Diethyl ether anesthesia induces transient cytosolic [Ca2+]
increase, heat shock proteins, and heat stress tolerance of
photosystem II in Arabidopsis

J 2022

Diethyl ether anesthesia induces transient cytosolic [Ca2+] increase, heat shock proteins, and heat stress tolerance of photosystem II in Arabidopsis

PAVLOVIC, Andrej, Jana JAKSOVA, Zuzana KUCEROVA, Martina SPUNDOVA, Marek RAC et. al.

Basic information

Original name

Diethyl ether anesthesia induces transient cytosolic [Ca2+] increase, heat shock proteins, and heat stress tolerance of photosystem II in Arabidopsis

Authors

PAVLOVIC, Andrej, Jana JAKSOVA, Zuzana KUCEROVA, Martina SPUNDOVA, Marek RAC, Pavel ROUDNICKÝ (203 Czech Republic, guarantor, belonging to the institution) and Axel MITHOFER

Edition

Frontiers in Plant Science, Lausanne, FRONTIERS MEDIA SA, 2022, 1664-462X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.600

RIV identification code

RIV/00216224:14740/22:00128520

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3389/fpls.2022.995001

UT WoS

000860606700001

Keywords in English

anesthesia; Arabidopsis; chlorophyll; diethyl ether; heat shock proteins; heat stress; photosystem II

Abstract

V originále

General volatile anesthetic diethyl ether blocks sensation and responsive behavior not only in animals but also in plants. Here, using a combination of RNA-seq and proteomic LC-MS/MS analyses, we investigated the effect of anesthetic diethyl ether on gene expression and downstream consequences in plant Arabidopsis thaliana. Differential expression analyses revealed reprogramming of gene expression under anesthesia: 6,168 genes were upregulated, 6,310 genes were downregulated, while 9,914 genes were not affected in comparison with control plants. On the protein level, out of 5,150 proteins identified, 393 were significantly upregulated and 227 were significantly downregulated. Among the highest significantly downregulated processes in etherized plants were chlorophyll/tetrapyrrole biosynthesis and photosynthesis. However, measurements of chlorophyll a fluorescence did not show inhibition of electron transport through photosystem II. The most significantly upregulated process was the response to heat stress (mainly heat shock proteins, HSPs). Using transgenic A. thaliana expressing APOAEQUORIN, we showed transient increase of cytoplasmic calcium level [Ca2+](cyt) in response to diethyl ether application. In addition, cell membrane permeability for ions also increased under anesthesia. The plants pre-treated with diethyl ether, and thus with induced HSPs, had increased tolerance of photosystem II to subsequent heat stress through the process known as cross-tolerance or priming. All these data indicate that diethyl ether anesthesia may partially mimic heat stress in plants through the effect on plasma membrane.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

PAVLOVIC, Andrej, Jana JAKSOVA, Zuzana KUCEROVA, Martina SPUNDOVA, Marek RAC, Pavel ROUDNICKÝ and Axel MITHOFER. Diethyl ether anesthesia induces transient cytosolic [Ca2+] increase, heat shock proteins, and heat stress tolerance of photosystem II in Arabidopsis. Frontiers in Plant Science. Lausanne: FRONTIERS MEDIA SA, 2022, vol. 13, SEP, p. 995001-995016. ISSN 1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2022.995001.

@article{2252400,
   author = {Pavlovic, Andrej and Jaksova, Jana and Kucerova, Zuzana and Spundova, Martina and Rac, Marek and Roudnický, Pavel and Mithofer, Axel},
   article_location = {Lausanne},
   article_number = {SEP},
   doi = {http://dx.doi.org/10.3389/fpls.2022.995001},
   keywords = {anesthesia; Arabidopsis; chlorophyll; diethyl ether; heat shock proteins; heat stress; photosystem II},
   language = {eng},
   issn = {1664-462X},
   journal = {Frontiers in Plant Science},
   title = {Diethyl ether anesthesia induces transient cytosolic [Ca2+] increase, heat shock proteins, and heat stress tolerance of photosystem II in Arabidopsis},
   url = {https://www.frontiersin.org/articles/10.3389/fpls.2022.995001/full},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2252400
AU  - Pavlovic, Andrej - Jaksova, Jana - Kucerova, Zuzana - Spundova, Martina - Rac, Marek - Roudnický, Pavel - Mithofer, Axel
PY  - 2022
TI  - Diethyl ether anesthesia induces transient cytosolic [Ca2+] increase, heat shock proteins, and heat stress tolerance of photosystem II in Arabidopsis
JF  - Frontiers in Plant Science
VL  - 13
IS  - SEP
SP  - 995001
EP  - 995001
PB  - FRONTIERS MEDIA SA
SN  - 1664462X
KW  - anesthesia
KW  - Arabidopsis
KW  - chlorophyll
KW  - diethyl ether
KW  - heat shock proteins
KW  - heat stress
KW  - photosystem II
UR  - https://www.frontiersin.org/articles/10.3389/fpls.2022.995001/full
N2  - General volatile anesthetic diethyl ether blocks sensation and responsive behavior not only in animals but also in plants. Here, using a combination of RNA-seq and proteomic LC-MS/MS analyses, we investigated the effect of anesthetic diethyl ether on gene expression and downstream consequences in plant Arabidopsis thaliana. Differential expression analyses revealed reprogramming of gene expression under anesthesia: 6,168 genes were upregulated, 6,310 genes were downregulated, while 9,914 genes were not affected in comparison with control plants. On the protein level, out of 5,150 proteins identified, 393 were significantly upregulated and 227 were significantly downregulated. Among the highest significantly downregulated processes in etherized plants were chlorophyll/tetrapyrrole biosynthesis and photosynthesis. However, measurements of chlorophyll a fluorescence did not show inhibition of electron transport through photosystem II. The most significantly upregulated process was the response to heat stress (mainly heat shock proteins, HSPs). Using transgenic A. thaliana expressing APOAEQUORIN, we showed transient increase of cytoplasmic calcium level [Ca2+](cyt) in response to diethyl ether application. In addition, cell membrane permeability for ions also increased under anesthesia. The plants pre-treated with diethyl ether, and thus with induced HSPs, had increased tolerance of photosystem II to subsequent heat stress through the process known as cross-tolerance or priming. All these data indicate that diethyl ether anesthesia may partially mimic heat stress in plants through the effect on plasma membrane.
ER  -

PAVLOVIC, Andrej, Jana JAKSOVA, Zuzana KUCEROVA, Martina SPUNDOVA, Marek RAC, Pavel ROUDNICKÝ and Axel MITHOFER. Diethyl ether anesthesia induces transient cytosolic [Ca2+] increase, heat shock proteins, and heat stress tolerance of photosystem II in Arabidopsis. \textit{Frontiers in Plant Science}. Lausanne: FRONTIERS MEDIA SA, 2022, vol.~13, SEP, p.~995001-995016. ISSN~1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2022.995001.

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