| ZAPLETAL, David, Eliska TABORSKA, Josef PASULKA, Radek MALIK, Karel KUBÍČEK, Martina ZÁNOVÁ, Christian MUCH, Marek ŠEBESTA, Valeria BUCCHERI, Filip HORVAT, Irena JENICKOVA, Michaela PROCHAZKOVA, Jan PROCHAZKA, Matyáš PINKAS, Jiří NOVÁČEK, Diego F JOSEPH, Radislav SEDLACEK, Carrie BERNECKY, Donal CARROLL, Richard ŠTEFL and Petr SVOBODA. Structural and functional basis of mammalian microRNA biogenesis by Dicer. Molecular Cell. CAMBRIDGE: CELL PRESS, 2022, vol. 82, No 21, p. 4064-4079. ISSN 1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2022.10.010. |
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@article{2252813,
author = {Zapletal, David and Taborska, Eliska and Pasulka, Josef and Malik, Radek and Kubíček, Karel and Zánová, Martina and Much, Christian and Šebesta, Marek and Buccheri, Valeria and Horvat, Filip and Jenickova, Irena and Prochazkova, Michaela and Prochazka, Jan and Pinkas, Matyáš and Nováček, Jiří and Joseph, Diego F and Sedlacek, Radislav and Bernecky, Carrie and Carroll, Donal and Štefl, Richard and Svoboda, Petr},
article_location = {CAMBRIDGE},
article_number = {21},
doi = {http://dx.doi.org/10.1016/j.molcel.2022.10.010},
keywords = {CRYO-EM STRUCTUREGUIDE STRAND SELECTIONRNA-BINDINGSTRUCTURE VALIDATIONTRBP COMPLEXMOUSEEXPRESSIONMOLPROBITYSPECIFICITYRECOGNITION},
language = {eng},
issn = {1097-2765},
journal = {Molecular Cell},
title = {Structural and functional basis of mammalian microRNA biogenesis by Dicer},
url = {https://www.sciencedirect.com/science/article/pii/S1097276522009674?via%3Dihub},
volume = {82},
year = {2022}
}
TY - JOUR
ID - 2252813
AU - Zapletal, David - Taborska, Eliska - Pasulka, Josef - Malik, Radek - Kubíček, Karel - Zánová, Martina - Much, Christian - Šebesta, Marek - Buccheri, Valeria - Horvat, Filip - Jenickova, Irena - Prochazkova, Michaela - Prochazka, Jan - Pinkas, Matyáš - Nováček, Jiří - Joseph, Diego F - Sedlacek, Radislav - Bernecky, Carrie - Carroll, Donal - Štefl, Richard - Svoboda, Petr
PY - 2022
TI - Structural and functional basis of mammalian microRNA biogenesis by Dicer
JF - Molecular Cell
VL - 82
IS - 21
SP - 4064-4079
EP - 4064-4079
PB - CELL PRESS
SN - 10972765
KW - CRYO-EM STRUCTUREGUIDE STRAND SELECTIONRNA-BINDINGSTRUCTURE VALIDATIONTRBP COMPLEXMOUSEEXPRESSIONMOLPROBITYSPECIFICITYRECOGNITION
UR - https://www.sciencedirect.com/science/article/pii/S1097276522009674?via%3Dihub
N2 - MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucle-ases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is spe-cifically adapted to miRNA biogenesis is unknown. We show that the adaptation entails a unique structural role of Dicer???s DExD/H helicase domain. Although mice tolerate loss of its putative ATPase function, the com-plete absence of the domain is lethal because it assures high-fidelity miRNA biogenesis. Structures of murine Dicerd???miRNA precursor complexes revealed that the DExD/H domain has a helicase-unrelated structural function. It locks Dicer in a closed state, which facilitates miRNA precursor selection. Transition to a cleav-age-competent open state is stimulated by Dicer-binding protein TARBP2. Absence of the DExD/H domain or its mutations unlocks the closed state, reduces substrate selectivity, and activates RNAi. Thus, the DExD/H domain structurally contributes to mammalian miRNA biogenesis and underlies mechanistical partitioning of miRNA and RNAi pathways.
ER -
ZAPLETAL, David, Eliska TABORSKA, Josef PASULKA, Radek MALIK, Karel KUBÍČEK, Martina ZÁNOVÁ, Christian MUCH, Marek ŠEBESTA, Valeria BUCCHERI, Filip HORVAT, Irena JENICKOVA, Michaela PROCHAZKOVA, Jan PROCHAZKA, Matyáš PINKAS, Jiří NOVÁČEK, Diego F JOSEPH, Radislav SEDLACEK, Carrie BERNECKY, Donal CARROLL, Richard ŠTEFL and Petr SVOBODA. Structural and functional basis of mammalian microRNA biogenesis by Dicer. \textit{Molecular Cell}. CAMBRIDGE: CELL PRESS, 2022, vol.~82, No~21, p.~4064-4079. ISSN~1097-2765. Available from: https://dx.doi.org/10.1016/j.molcel.2022.10.010.
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