Water potential governs the effector specificity of the
transcriptional regulator XylR of Pseudomonas putida

J 2023

Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida

DVOŘÁK, Pavel; Teca Calcagno GALVÃO; Katharina PFLÜGER-GRAU; Alice M. BANKS; Víctor DE LORENZO et. al.

Základní údaje

Originální název

Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida

Autoři

DVOŘÁK, Pavel (203 Česká republika, domácí); Teca Calcagno GALVÃO; Katharina PFLÜGER-GRAU; Alice M. BANKS; Víctor DE LORENZO (garant) a Jose I. JIMÉNEZ

Vydání

Environmental Microbiology, Wiley, 2023, 1462-2912

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300

Kód RIV

RIV/00216224:14310/23:00134057

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1111/1462-2920.16342

UT WoS

000924226000001

EID Scopus

2-s2.0-85147337278

Klíčová slova anglicky

Pseudomonas putida; bacteria; genetic engineering; protein-structure; range; activation; sequence

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 6. 2023 15:53, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The biodegradative capacity of bacteria in their natural habitats is affected by water availability. In this work, we have examined the activity and effector specificity of the transcriptional regulator XylR of the TOL plasmid pWW0 of Pseudomonas putida mt-2 for biodegradation of m-xylene when external water potential was manipulated with polyethylene glycol PEG8000. By using non-disruptive luxCDEAB reporter technology, we noticed that the promoter activated by XylR (Pu) restricted its activity and the regulator became more effector-specific towards head TOL substrates when cells were grown under water subsaturation. Such a tight specificity brought about by water limitation was relaxed when intracellular osmotic stress was counteracted by the external addition of the compatible solute glycine betaine. With these facts in hand, XylR variants isolated earlier as effector-specificity responders to the non-substrate 1,2,4-trichlorobenzene under high matric stress were re-examined and found to be unaffected by water potential in vivo. All these phenomena could be ultimately explained as the result of water potential-dependent conformational changes in the A domain of XylR and its effector-binding pocket, as suggested by AlphaFold prediction of protein structures. The consequences of this scenario for the evolution of specificities in regulators and the emergence of catabolic pathways are discussed.

Návaznosti

GA22-10845S, projekt VaV

Název: Studium role polyhydroxyalkanoátů u bakterie Schlegelella thermodepolymerans – slibného bakteriálního kandidáta pro biotechnologie nové generace (Akronym: PHAST)

Investor: Grantová agentura ČR, Studium role polyhydroxyalkanoátů u bakterie Schlegelella thermodepolymerans – slibného bakteriálního kandidáta pro biotechnologie nové generace

Citovat

DVOŘÁK, Pavel; Teca Calcagno GALVÃO; Katharina PFLÜGER-GRAU; Alice M. BANKS; Víctor DE LORENZO a Jose I. JIMÉNEZ. Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida. Environmental Microbiology. Wiley, 2023, roč. 25, č. 5, s. 1041-1054. ISSN 1462-2912. Dostupné z: https://doi.org/10.1111/1462-2920.16342.

@article{2253366,
   author = {Dvořák, Pavel and Galvão, Teca Calcagno and PflügerandGrau, Katharina and Banks, Alice M. and De Lorenzo, Víctor and Jiménez, Jose I.},
   article_number = {5},
   doi = {https://doi.org/10.1111/1462-2920.16342},
   keywords = {Pseudomonas putida; bacteria; genetic engineering; protein-structure; range; activation; sequence},
   language = {eng},
   issn = {1462-2912},
   journal = {Environmental Microbiology},
   title = {Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida},
   url = {https://doi.org/10.1111/1462-2920.16342},
   volume = {25},
   year = {2023}
}

TY  - JOUR
ID  - 2253366
AU  - Dvořák, Pavel - Galvão, Teca Calcagno - Pflüger-Grau, Katharina - Banks, Alice M. - De Lorenzo, Víctor - Jiménez, Jose I.
PY  - 2023
TI  - Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida
JF  - Environmental Microbiology
VL  - 25
IS  - 5
SP  - 1041-1054
EP  - 1041-1054
PB  - Wiley
SN  - 14622912
KW  - Pseudomonas putida
KW  - bacteria
KW  - genetic engineering
KW  - protein-structure
KW  - range
KW  - activation
KW  - sequence
UR  - https://doi.org/10.1111/1462-2920.16342
N2  - The biodegradative capacity of bacteria in their natural habitats is affected by water availability. In this work, we have examined the activity and effector specificity of the transcriptional regulator XylR of the TOL plasmid pWW0 of Pseudomonas putida mt-2 for biodegradation of m-xylene when external water potential was manipulated with polyethylene glycol PEG8000. By using non-disruptive luxCDEAB reporter technology, we noticed that the promoter activated by XylR (Pu) restricted its activity and the regulator became more effector-specific towards head TOL substrates when cells were grown under water subsaturation. Such a tight specificity brought about by water limitation was relaxed when intracellular osmotic stress was counteracted by the external addition of the compatible solute glycine betaine. With these facts in hand, XylR variants isolated earlier as effector-specificity responders to the non-substrate 1,2,4-trichlorobenzene under high matric stress were re-examined and found to be unaffected by water potential in vivo. All these phenomena could be ultimately explained as the result of water potential-dependent conformational changes in the A domain of XylR and its effector-binding pocket, as suggested by AlphaFold prediction of protein structures. The consequences of this scenario for the evolution of specificities in regulators and the emergence of catabolic pathways are discussed.
ER  -

DVOŘÁK, Pavel; Teca Calcagno GALVÃO; Katharina PFLÜGER-GRAU; Alice M. BANKS; Víctor DE LORENZO a Jose I. JIMÉNEZ. Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida. \textit{Environmental Microbiology}. Wiley, 2023, roč.~25, č.~5, s.~1041-1054. ISSN~1462-2912. Dostupné z: https://doi.org/10.1111/1462-2920.16342.

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