J 2023

Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida

DVOŘÁK, Pavel, Teca Calcagno GALVÃO, Katharina PFLÜGER-GRAU, Alice M. BANKS, Víctor DE LORENZO et. al.

Basic information

Original name

Water potential governs the effector specificity of the transcriptional regulator XylR of Pseudomonas putida

Authors

DVOŘÁK, Pavel (203 Czech Republic, belonging to the institution), Teca Calcagno GALVÃO, Katharina PFLÜGER-GRAU, Alice M. BANKS, Víctor DE LORENZO (guarantor) and Jose I. JIMÉNEZ

Edition

Environmental Microbiology, Wiley, 2023, 1462-2912

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.100 in 2022

RIV identification code

RIV/00216224:14310/23:00134057

Organization unit

Faculty of Science

DOI

UT WoS

000924226000001

Keywords in English

Pseudomonas putida; bacteria; genetic engineering; protein-structure; range; activation; sequence

Tags

Tags

International impact, Reviewed
Změněno: 21/6/2023 15:53, Mgr. Marie Šípková, DiS.

Abstract

V originále

The biodegradative capacity of bacteria in their natural habitats is affected by water availability. In this work, we have examined the activity and effector specificity of the transcriptional regulator XylR of the TOL plasmid pWW0 of Pseudomonas putida mt-2 for biodegradation of m-xylene when external water potential was manipulated with polyethylene glycol PEG8000. By using non-disruptive luxCDEAB reporter technology, we noticed that the promoter activated by XylR (Pu) restricted its activity and the regulator became more effector-specific towards head TOL substrates when cells were grown under water subsaturation. Such a tight specificity brought about by water limitation was relaxed when intracellular osmotic stress was counteracted by the external addition of the compatible solute glycine betaine. With these facts in hand, XylR variants isolated earlier as effector-specificity responders to the non-substrate 1,2,4-trichlorobenzene under high matric stress were re-examined and found to be unaffected by water potential in vivo. All these phenomena could be ultimately explained as the result of water potential-dependent conformational changes in the A domain of XylR and its effector-binding pocket, as suggested by AlphaFold prediction of protein structures. The consequences of this scenario for the evolution of specificities in regulators and the emergence of catabolic pathways are discussed.

Links

GA22-10845S, research and development project
Name: Studium role polyhydroxyalkanoátů u bakterie Schlegelella thermodepolymerans – slibného bakteriálního kandidáta pro biotechnologie nové generace (Acronym: PHAST)
Investor: Czech Science Foundation, Unraveling the role of polyhydroxyalkanoates in Schlegelella thermodepolymerans – promising environmental bacterium for next generation biotechnology