| BINÓ, Lucia, Erika MIKULENKOVÁ, Luděk ŠTĚPÁNEK, Ondřej BERNATÍK, David VYSLOUŽIL, Petra PEJŠKOVÁ, Peter GORILÁK, Martina HURANOVÁ, Vadimír VARGA and Lukáš ČAJÁNEK. A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines. STAR Protocols. United States: Cell Press, 2022, vol. 3, No 1, p. 1-33. ISSN 2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2022.101199. |
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@article{2253640,
author = {Binó, Lucia and Mikulenková, Erika and Štěpánek, Luděk and Bernatík, Ondřej and Vysloužil, David and Pejšková, Petra and Gorilák, Peter and Huranová, Martina and Varga, Vadimír and Čajánek, Lukáš},
article_location = {United States},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.xpro.2022.101199},
keywords = {Cell Biology; Cell culture; Microscopy; Molecular Biology},
language = {eng},
issn = {2666-1667},
journal = {STAR Protocols},
title = {A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines},
url = {https://www.sciencedirect.com/science/article/pii/S266616672200079X},
volume = {3},
year = {2022}
}
TY - JOUR
ID - 2253640
AU - Binó, Lucia - Mikulenková, Erika - Štěpánek, Luděk - Bernatík, Ondřej - Vysloužil, David - Pejšková, Petra - Gorilák, Peter - Huranová, Martina - Varga, Vadimír - Čajánek, Lukáš
PY - 2022
TI - A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
JF - STAR Protocols
VL - 3
IS - 1
SP - 1-33
EP - 1-33
PB - Cell Press
SN - 26661667
KW - Cell Biology
KW - Cell culture
KW - Microscopy
KW - Molecular Biology
UR - https://www.sciencedirect.com/science/article/pii/S266616672200079X
N2 - Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).
ER -
BINÓ, Lucia, Erika MIKULENKOVÁ, Luděk ŠTĚPÁNEK, Ondřej BERNATÍK, David VYSLOUŽIL, Petra PEJŠKOVÁ, Peter GORILÁK, Martina HURANOVÁ, Vadimír VARGA and Lukáš ČAJÁNEK. A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines. \textit{STAR Protocols}. United States: Cell Press, 2022, vol.~3, No~1, p.~1-33. ISSN~2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2022.101199.
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