A protocol for generation and live-cell imaging analysis of
primary cilia reporter cell lines

J 2022

A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

BINÓ, Lucia, Erika MIKULENKOVÁ, Luděk ŠTĚPÁNEK, Ondřej BERNATÍK, David VYSLOUŽIL et. al.

Basic information

Original name

A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines

Authors

BINÓ, Lucia (703 Slovakia, belonging to the institution), Erika MIKULENKOVÁ (203 Czech Republic, belonging to the institution), Luděk ŠTĚPÁNEK (203 Czech Republic), Ondřej BERNATÍK (203 Czech Republic, belonging to the institution), David VYSLOUŽIL (203 Czech Republic, belonging to the institution), Petra PEJŠKOVÁ (203 Czech Republic, belonging to the institution), Peter GORILÁK, Martina HURANOVÁ, Vadimír VARGA and Lukáš ČAJÁNEK (203 Czech Republic, belonging to the institution)

Edition

STAR Protocols, United States, Cell Press, 2022, 2666-1667

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14110/22:00128587

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.xpro.2022.101199

UT WoS

001141790100017

Keywords in English

Cell Biology; Cell culture; Microscopy; Molecular Biology

Abstract

V originále

Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).

Links

GA19-05244S, research and development project

Name: Molekulární aspekty ciliogeneze: zaměření na Tau Tubulin Kinázu 2

Investor: Czech Science Foundation

GA21-21612S, research and development project

Name: Souhra mezi regulacemi buněčného cyklu a ciliogeneze

Investor: Czech Science Foundation

GA22-13277S, research and development project

Name: Ciliární Tau tubulin kináza 2 ve zdraví a nemoci: struktura a funkce

Investor: Czech Science Foundation

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BINÓ, Lucia, Erika MIKULENKOVÁ, Luděk ŠTĚPÁNEK, Ondřej BERNATÍK, David VYSLOUŽIL, Petra PEJŠKOVÁ, Peter GORILÁK, Martina HURANOVÁ, Vadimír VARGA and Lukáš ČAJÁNEK. A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines. STAR Protocols. United States: Cell Press, 2022, vol. 3, No 1, p. 1-33. ISSN 2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2022.101199.

@article{2253640,
   author = {Binó, Lucia and Mikulenková, Erika and Štěpánek, Luděk and Bernatík, Ondřej and Vysloužil, David and Pejšková, Petra and Gorilák, Peter and Huranová, Martina and Varga, Vadimír and Čajánek, Lukáš},
   article_location = {United States},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.xpro.2022.101199},
   keywords = {Cell Biology; Cell culture; Microscopy; Molecular Biology},
   language = {eng},
   issn = {2666-1667},
   journal = {STAR Protocols},
   title = {A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines},
   url = {https://www.sciencedirect.com/science/article/pii/S266616672200079X},
   volume = {3},
   year = {2022}
}

TY  - JOUR
ID  - 2253640
AU  - Binó, Lucia - Mikulenková, Erika - Štěpánek, Luděk - Bernatík, Ondřej - Vysloužil, David - Pejšková, Petra - Gorilák, Peter - Huranová, Martina - Varga, Vadimír - Čajánek, Lukáš
PY  - 2022
TI  - A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines
JF  - STAR Protocols
VL  - 3
IS  - 1
SP  - 1-33
EP  - 1-33
PB  - Cell Press
SN  - 26661667
KW  - Cell Biology
KW  - Cell culture
KW  - Microscopy
KW  - Molecular Biology
UR  - https://www.sciencedirect.com/science/article/pii/S266616672200079X
N2  - Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).
ER  -

BINÓ, Lucia, Erika MIKULENKOVÁ, Luděk ŠTĚPÁNEK, Ondřej BERNATÍK, David VYSLOUŽIL, Petra PEJŠKOVÁ, Peter GORILÁK, Martina HURANOVÁ, Vadimír VARGA and Lukáš ČAJÁNEK. A protocol for generation and live-cell imaging analysis of primary cilia reporter cell lines. \textit{STAR Protocols}. United States: Cell Press, 2022, vol.~3, No~1, p.~1-33. ISSN~2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2022.101199.

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