Compromised function of ARM, the interactor of Arabidopsis
telomerase, suggests its role in stress responses

J 2022

Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses

PŘIKRYLOVÁ, Klára, Agata Magdalena KILAR, Petra KOVÁČIKOVÁ, Jiří FAJKUS, Eva SÝKOROVÁ et. al.

Basic information

Original name

Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses

Authors

PŘIKRYLOVÁ, Klára (203 Czech Republic, belonging to the institution), Agata Magdalena KILAR (616 Poland, belonging to the institution), Petra KOVÁČIKOVÁ (703 Slovakia, belonging to the institution), Jiří FAJKUS (203 Czech Republic, belonging to the institution), Eva SÝKOROVÁ and Miloslava FOJTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Science, Elsevier, 2022, 0168-9452

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.200

RIV identification code

RIV/00216224:14310/22:00128593

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.plantsci.2022.111453

UT WoS

000862382300002

Keywords in English

ARM; Arabidopsis thaliana; Armadillo; ?-catenin-like repeat; RNAseq; Stress response; Telomerase

Abstract

V originále

ARM was identified previously as an interaction partner of the telomerase protein subunit (TERT) in Arabidopsis thaliana. To investigate the interconnection between ARM and telomerase and to identify ARM cellular functions, we analyzed a set of arm mutant lines and arm/tert double mutants. Telomere length was not affected in arm single mutant plants, in contrast to double mutants. In the second generation of homozygous arm-1/tert double mutants following the heterozygous state during the double mutant construction, telomeres shortened dramat-ically, even below levels in tert plants displaying severe morphological defects. Intriguingly, homozygous arm-1/ tert double mutants with short telomeres grew without obvious phenotypic changes for next two generations. Then, in agreement with the onset of phenotypic changes in tert, morphological defects were timed to the 5th arm-1/tert homozygous generation. RNAseq analyses of arm-1/tert and respective single mutants displayed markedly overlapping sets of differentially expressed genes in arm-1/tert double mutant and arm-1 single mutant lines, indicating a dominant effect of the ARM mutation. RNAseq data further implied ARM involvement in circadian rhythms, responses to drugs and to biotic and abiotic stimuli. In agreement with it, we observed sensitivity of arm-1 single mutant to the heat stress during germination. Altogether, our results suggest ARM involvement in crucial cellular processes without evidencing its role in the telomerase canonical function.

Links

EF16_026/0008446, research and development project

Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

PŘIKRYLOVÁ, Klára, Agata Magdalena KILAR, Petra KOVÁČIKOVÁ, Jiří FAJKUS, Eva SÝKOROVÁ and Miloslava FOJTOVÁ. Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses. Plant Science. Elsevier, 2022, vol. 325, December, p. 1-16. ISSN 0168-9452. Available from: https://dx.doi.org/10.1016/j.plantsci.2022.111453.

@article{2253727,
   author = {Přikrylová, Klára and Kilar, Agata Magdalena and Kováčiková, Petra and Fajkus, Jiří and Sýkorová, Eva and Fojtová, Miloslava},
   article_number = {December},
   doi = {http://dx.doi.org/10.1016/j.plantsci.2022.111453},
   keywords = {ARM; Arabidopsis thaliana; Armadillo; ?-catenin-like repeat; RNAseq; Stress response; Telomerase},
   language = {eng},
   issn = {0168-9452},
   journal = {Plant Science},
   title = {Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses},
   url = {https://www.sciencedirect.com/science/article/pii/S0168945222002783?via%3Dihub},
   volume = {325},
   year = {2022}
}

TY  - JOUR
ID  - 2253727
AU  - Přikrylová, Klára - Kilar, Agata Magdalena - Kováčiková, Petra - Fajkus, Jiří - Sýkorová, Eva - Fojtová, Miloslava
PY  - 2022
TI  - Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses
JF  - Plant Science
VL  - 325
IS  - December
SP  - 1-16
EP  - 1-16
PB  - Elsevier
SN  - 01689452
KW  - ARM
KW  - Arabidopsis thaliana
KW  - Armadillo
KW  - ?-catenin-like repeat
KW  - RNAseq
KW  - Stress response
KW  - Telomerase
UR  - https://www.sciencedirect.com/science/article/pii/S0168945222002783?via%3Dihub
N2  - ARM was identified previously as an interaction partner of the telomerase protein subunit (TERT) in Arabidopsis thaliana. To investigate the interconnection between ARM and telomerase and to identify ARM cellular functions, we analyzed a set of arm mutant lines and arm/tert double mutants. Telomere length was not affected in arm single mutant plants, in contrast to double mutants. In the second generation of homozygous arm-1/tert double mutants following the heterozygous state during the double mutant construction, telomeres shortened dramat-ically, even below levels in tert plants displaying severe morphological defects. Intriguingly, homozygous arm-1/ tert double mutants with short telomeres grew without obvious phenotypic changes for next two generations. Then, in agreement with the onset of phenotypic changes in tert, morphological defects were timed to the 5th arm-1/tert homozygous generation. RNAseq analyses of arm-1/tert and respective single mutants displayed markedly overlapping sets of differentially expressed genes in arm-1/tert double mutant and arm-1 single mutant lines, indicating a dominant effect of the ARM mutation. RNAseq data further implied ARM involvement in circadian rhythms, responses to drugs and to biotic and abiotic stimuli. In agreement with it, we observed sensitivity of arm-1 single mutant to the heat stress during germination. Altogether, our results suggest ARM involvement in crucial cellular processes without evidencing its role in the telomerase canonical function.
ER  -

PŘIKRYLOVÁ, Klára, Agata Magdalena KILAR, Petra KOVÁČIKOVÁ, Jiří FAJKUS, Eva SÝKOROVÁ and Miloslava FOJTOVÁ. Compromised function of ARM, the interactor of Arabidopsis telomerase, suggests its role in stress responses. \textit{Plant Science}. Elsevier, 2022, vol.~325, December, p.~1-16. ISSN~0168-9452. Available from: https://dx.doi.org/10.1016/j.plantsci.2022.111453.

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