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@article{2259397,
author = {Krausova, Katerina and Charousova, Marketa and Kratochvil, Zdenek and Takacsova, Paulina and Tesarova, Barbora and Sivak, Ladislav and Kudličková Pešková, Marie and Sukupova, Martina and Zivotska, Hana and Makovicky, Pavol and Yamashita, Ichiro and Okamoto, Naofumi and Hynek, David and Haddad, Yazan and Pekařík, Vladimír and Rex, Simona and Heger, Zbynek},
article_location = {Amsterdam},
article_number = {AUG},
doi = {http://dx.doi.org/10.1016/j.apmt.2022.101535},
keywords = {Archaeal ferritin; Continuous multimodal nanoparticle size; analysis; Mammalian ferritin; Native PAGE; Protein corona},
language = {eng},
issn = {2352-9407},
journal = {Applied Materials Today},
title = {Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition},
url = {https://www.sciencedirect.com/science/article/pii/S2352940722001706?via%3Dihub},
volume = {28},
year = {2022}
}
TY - JOUR
ID - 2259397
AU - Krausova, Katerina - Charousova, Marketa - Kratochvil, Zdenek - Takacsova, Paulina - Tesarova, Barbora - Sivak, Ladislav - Kudličková Pešková, Marie - Sukupova, Martina - Zivotska, Hana - Makovicky, Pavol - Yamashita, Ichiro - Okamoto, Naofumi - Hynek, David - Haddad, Yazan - Pekařík, Vladimír - Rex, Simona - Heger, Zbynek
PY - 2022
TI - Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition
JF - Applied Materials Today
VL - 28
IS - AUG
SP - 101535
EP - 101535
PB - Elsevier
SN - 23529407
KW - Archaeal ferritin
KW - Continuous multimodal nanoparticle size
KW - analysis
KW - Mammalian ferritin
KW - Native PAGE
KW - Protein corona
UR - https://www.sciencedirect.com/science/article/pii/S2352940722001706?via%3Dihub
N2 - Understanding the reversible reassembly properties of ferritins (FRTs) is crucial for enabling their applicability in nanomedicine. These properties include drug loading capabilities and also subsequent payload release in desired site-of-action. Thus, the presented study is focused on understanding the disassembly of recombinantly produced FRTs of varying origin and subunit composition, i.e. equine FRT composed of 22 L- and 2 H-subunits (EcaLH) or 24 L-subunits (EcaL), human FRT composed of 24 H-subunits (HsaH) and archaeal Pyrococcus furiosus FRT (Pfu). Disassembly was distinctly influenced by pH and ionic strength. Except for Pfu, the disassembly kinetics in acidic pH is rapid upon reaching an innate threshold, reaching the final state within minutes. Disassembly kinetics in basic pH is slower. Pfu is partially disassembled within the entire pH range. While equine FRT disassembles into free subunits, HsaH disassembles into subunit clusters. EcaL and Pfu form large aggregates during disassembly in mildly acidic pH, although basic pH causes partial disassembly without aggregation, suggesting usability for basic pH-triggered drug loading. We show that in human serum/plasma, FRTs readily form protein corona, hampering their uptake. Interestingly, we found out that archaeal Pfu likely exhibits similar receptor affinity as mammalian FRTs. Further, in vivo toxicity and biodistribution study of a single dose of FRTs demonstrated very low toxicity of FRTs and their preferential liver/kidney bioaccumulation.
ER -
KRAUSOVA, Katerina, Marketa CHAROUSOVA, Zdenek KRATOCHVIL, Paulina TAKACSOVA, Barbora TESAROVA, Ladislav SIVAK, Marie KUDLIČKOVÁ PEŠKOVÁ, Martina SUKUPOVA, Hana ZIVOTSKA, Pavol MAKOVICKY, Ichiro YAMASHITA, Naofumi OKAMOTO, David HYNEK, Yazan HADDAD, Vladimír PEKAŘÍK, Simona REX and Zbynek HEGER. Toward understanding the kinetics of disassembly of ferritins of varying origin and subunit composition. \textit{Applied Materials Today}. Amsterdam: Elsevier, 2022, vol.~28, AUG, p.~101535-101546. ISSN~2352-9407. Available from: https://dx.doi.org/10.1016/j.apmt.2022.101535.
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