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@article{2261737,
author = {Knowles, J. and Boryshpolets, S. and Kholodnyy, V. and Rahi, D. and Vysloužil, Jakub and Muselík, Jan and Stejskal, V. and Kouril, J. and Podhorec, P.},
article_location = {Amsterdam},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.animal.2021.100430},
keywords = {Fish; Maturation; Sperm motility; Sperm production; Sustained drug release},
language = {eng},
issn = {1751-7311},
journal = {ANIMAL},
title = {Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike},
url = {https://www.sciencedirect.com/science/article/pii/S1751731121002767?via%3Dihub},
volume = {16},
year = {2022}
}
TY - JOUR
ID - 2261737
AU - Knowles, J. - Boryshpolets, S. - Kholodnyy, V. - Rahi, D. - Vysloužil, Jakub - Muselík, Jan - Stejskal, V. - Kouril, J. - Podhorec, P.
PY - 2022
TI - Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike
JF - ANIMAL
VL - 16
IS - 1
SP - 1-8
EP - 1-8
PB - Elsevier
SN - 17517311
KW - Fish
KW - Maturation
KW - Sperm motility
KW - Sperm production
KW - Sustained drug release
UR - https://www.sciencedirect.com/science/article/pii/S1751731121002767?via%3Dihub
N2 - Artificial reproduction of northern pike Esox lucius is impeded by the likelihood of obtaining only a small volume of sperm of inconsistent quality. A controlled-release hormone delivery system has the potential to enhance sperm production while avoiding multiple injections The objective of this study was to investigate the effects of mammalian gonadotropin-releasing hormone agonist (mGnRHa) incorporated into poly(lactic-co-glycolic acid) (PLGA) microparticles on milt production, spermatozoon characteristics, and secretion of 17β-estradiol (E2), 11-keto testosterone (11-KT), and testosterone in northern pike. Fish were divided into four groups and injected with 2 mg/kg BW carp pituitary extract (CPE), 20 µg/kg BW mGnRHa in PLGA microparticles, or 20 µg/kg BW mGnRHa plus 20 mg/kg BW metoclopramide (MET) in PLGA microparticles (PLGA + MET), along with a control group injected with 1 ml/kg 0.9% NaCl. At 48 h postinjection, the volume of milt produced was significantly greater in groups treated with CPE and PLGA + MET than in other groups. At 96 h postinjection, all hormone-treated groups exhibited significantly higher spermatozoon average velocity than recorded in the control group. Spermatozoon motility was significantly increased (P < 0.05) in the CPE and PLGA groups compared to baseline values. All treated groups showed significantly lower levels of 11-KT after the hormone injection compared to baseline values and to controls. Plasma testosterone levels increased in all hormone-treated groups. The use of PLGA microparticles, with or without metoclopramide, is suitable for use as a carrier of hormone treatments to regulate spermiation in mature northern pike.
ER -
KNOWLES, J., S. BORYSHPOLETS, V. KHOLODNYY, D. RAHI, Jakub VYSLOUŽIL, Jan MUSELÍK, V. STEJSKAL, J. KOURIL and P. PODHOREC. Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike. \textit{ANIMAL}. Amsterdam: Elsevier, 2022, vol.~16, No~1, p.~1-8. ISSN~1751-7311. Available from: https://dx.doi.org/10.1016/j.animal.2021.100430.
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