Single Cerebral Organoid Mass Spectrometry of Cell-Specific Protein and Glycosphingolipid Traits

NEZVEDOVÁ, Markéta, Durga JHA, Tereza VÁŇOVÁ, Darshak Chandulal GADARA, Hana KLÍMOVÁ, Jan RAŠKA, Lukáš OPÁLKA, Dáša BOHAČIAKOVÁ and Zdeněk SPÁČIL. Single Cerebral Organoid Mass Spectrometry of Cell-Specific Protein and Glycosphingolipid Traits. Analytical Chemistry. American Chemical Society, 2023, vol. 95, No 6, p. 3160-3167. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.2c00981.

Basic information

Original name

Single Cerebral Organoid Mass Spectrometry of Cell-Specific Protein and Glycosphingolipid Traits

Authors

NEZVEDOVÁ, Markéta (203 Czech Republic, belonging to the institution), Durga JHA (356 India, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution), Darshak Chandulal GADARA (356 India, belonging to the institution), Hana KLÍMOVÁ (203 Czech Republic, belonging to the institution), Jan RAŠKA (203 Czech Republic, belonging to the institution), Lukáš OPÁLKA, Dáša BOHAČIAKOVÁ (703 Slovakia, belonging to the institution) and Zdeněk SPÁČIL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical Chemistry, American Chemical Society, 2023, 0003-2700

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

DOI

Impact factor

Impact factor: 7.400 in 2022

RIV identification code

RIV/00216224:14310/23:00130378

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acs.analchem.2c00981

UT WoS

000927047200001

Keywords in English

Assays; Cells; Differentiation; Lipids; Peptides and proteins

Tags

14110517, rivok

Tags

International impact, Reviewed

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Abstract

V originále

Cerebral organoids are a prolific research topic and an emerging model system for neurological diseases in human neurobiology. However, the batch-to-batch reproducibility of current cultivation protocols is challenging and thus requires a high-throughput methodology to comprehensively characterize cerebral organoid cytoarchitecture and neural development. We report a mass spectrometry-based protocol to quantify neural tissue cell markers, cell surface lipids, and housekeeping proteins in a single organoid. Profiled traits probe the development of neural stem cells, radial glial cells, neurons, and astrocytes. We assessed the cell population heterogeneity in individually profiled organoids in the early and late neurogenesis stages. Here, we present a unifying view of cell-type specificity of profiled protein and lipid traits in neural tissue. Our workflow characterizes the cytoarchitecture, differentiation stage, and batch cultivation variation on an individual cerebral organoid level.

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Links

EF17_043/0009632, research and development project	Name: CETOCOEN Excellence
GA20-15728S, research and development project	Name: Nové přístupy k překonání terapeutické rezistence u glioblastoma multiforme se zaměřením na buněčnou senescenci a model mozkového organoidu Investor: Czech Science Foundation
GA21-21510S, research and development project	Name: Studium molekulárních mechanismů vzniku Alzheimerovy choroby pomocí cerebrálních organoidů (Acronym: AD Brain Organoids) Investor: Czech Science Foundation
GJ18-25429Y, research and development project	Name: Funkční studie mikroRNA u nerálních kmenových buněk v průběhu diferenciace Investor: Czech Science Foundation
LM2018121, research and development project	Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI) Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI
MUNI/G/1131/2017, interní kód MU	Name: Transformative stem cell-based model of Alzheimer’s disease and advanced analytics to study the role of membrane lipids in the pathogenesis Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects
MUNI/R/1697/2020, interní kód MU	Name: Career Restart - Stem Cell-Based Models of Neurodegeneration Investor: Masaryk University, CAREER RESTART
NV19-08-00472, research and development project	Name: Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí (Acronym: BioMAD) Investor: Ministry of Health of the CR
ROZV/23/LF/2019, interní kód MU	Name: LF - Příspěvek na IP 2019 Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
ROZV/28/LF/2020, interní kód MU	Name: LF - Příspěvek na InstP 2020 Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
857560, interní kód MU (CEP code: EF17_043/0009632)	Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence) Investor: European Union, Spreading excellence and widening participation

Files attached

2261917.pdf

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