The structured organization of Deinococcus radiodurans' cell
envelope

J 2022

The structured organization of Deinococcus radiodurans' cell envelope

FARCI, Domenica, Patrycja HANIEWICZ and Dario PIANO

Basic information

Original name

The structured organization of Deinococcus radiodurans' cell envelope

Authors

FARCI, Domenica, Patrycja HANIEWICZ and Dario PIANO

Edition

Proceedings of the National Academy of Sciences of the United States of America, 2022, 0027-8424

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.100

RIV identification code

RIV/00216224:14740/22:00128752

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1073/pnas.2209111119

UT WoS

000907643500039

Keywords in English

cryo-electron crystallography; cryo-electron tomography; S-layer; Type IV piliation system; SDBC

Abstract

V originále

Surface layers (S-layers) are highly ordered coats of proteins localized on the cell surface of many bacterial species. In these structures, one or more proteins form elementary units that self-assemble into a crystalline monolayer tiling the entire cell surface. Here, the cell envelope of the radiation-resistant bacterium Deinococcus radiodurans was studied by cryo-electron microscopy, finding the crystalline regularity of the S-layer extended into the layers below (outer membrane, periplasm, and inner membrane). The cell envelope appears to be highly packed and resulting from a three-dimensional crystalline distribution of protein complexes organized in close continuity yet allowing a certain degree of free space. The presented results suggest how S-layers, at least in some species, are mesoscale assemblies behaving as structural and functional scaffolds essential for the entire cell envelope.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

FARCI, Domenica, Patrycja HANIEWICZ and Dario PIANO. The structured organization of Deinococcus radiodurans' cell envelope. Proceedings of the National Academy of Sciences of the United States of America. 2022, vol. 119, No 45, p. 1-8. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.2209111119.

@article{2262398,
   author = {Farci, Domenica and Haniewicz, Patrycja and Piano, Dario},
   article_number = {45},
   doi = {http://dx.doi.org/10.1073/pnas.2209111119},
   keywords = {cryo-electron crystallography; cryo-electron tomography; S-layer; Type IV piliation system; SDBC},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {The structured organization of Deinococcus radiodurans' cell envelope},
   url = {https://www.pnas.org/doi/10.1073/pnas.2209111119},
   volume = {119},
   year = {2022}
}

TY  - JOUR
ID  - 2262398
AU  - Farci, Domenica - Haniewicz, Patrycja - Piano, Dario
PY  - 2022
TI  - The structured organization of Deinococcus radiodurans' cell envelope
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 119
IS  - 45
SP  - 1-8
EP  - 1-8
SN  - 00278424
KW  - cryo-electron crystallography
KW  - cryo-electron tomography
KW  - S-layer
KW  - Type IV piliation system
KW  - SDBC
UR  - https://www.pnas.org/doi/10.1073/pnas.2209111119
N2  - Surface layers (S-layers) are highly ordered coats of proteins localized on the cell surface of many bacterial species. In these structures, one or more proteins form elementary units that self-assemble into a crystalline monolayer tiling the entire cell surface. Here, the cell envelope of the radiation-resistant bacterium Deinococcus radiodurans was studied by cryo-electron microscopy, finding the crystalline regularity of the S-layer extended into the layers below (outer membrane, periplasm, and inner membrane). The cell envelope appears to be highly packed and resulting from a three-dimensional crystalline distribution of protein complexes organized in close continuity yet allowing a certain degree of free space. The presented results suggest how S-layers, at least in some species, are mesoscale assemblies behaving as structural and functional scaffolds essential for the entire cell envelope.
ER  -

FARCI, Domenica, Patrycja HANIEWICZ and Dario PIANO. The structured organization of Deinococcus radiodurans' cell envelope. \textit{Proceedings of the National Academy of Sciences of the United States of America}. 2022, vol.~119, No~45, p.~1-8. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.2209111119.

Detailed Information on Publication Record