Combination of Hypotonic Lysis and Application of Detergent for
Isolation of Polyhydroxyalkanoates from Extremophiles

J 2022

Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles

NOVACKOVA, Ivana, Xenie KOURILOVA, Katerina MRAZOVA, Petr SEDLACEK, Michal KALINA et. al.

Basic information

Original name

Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles

Authors

NOVACKOVA, Ivana, Xenie KOURILOVA, Katerina MRAZOVA, Petr SEDLACEK, Michal KALINA, Vladislav KRZYZANEK, Martin KOLLER and Stanislav OBRUCA

Edition

Polymers, MDPI, 2022, 2073-4360

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10404 Polymer science

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.000

RIV identification code

RIV/00216224:14740/22:00128758

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/polym14091761

UT WoS

000800729600001

Keywords in English

polyhydroxyalkanoate (PHA); sodium dodecyl sulfate (SDS); PHA isolation; extremophiles; halophiles; thermophiles; Halomonas halophila; Schlegelella thermodepolymerans

Abstract

V originále

Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage of the natural susceptibility of halophilic and thermophilic PHA producers to hypotonic lysis and we developed a simple and robust approach enabling effective isolation of PHA materials from microbial cells. The method is based on the exposition of microbial cells to hypotonic conditions induced by the diluted solution of sodium dodecyl sulfate (SDS) at elevated temperatures. Such conditions lead to disruption of the cells and release of PHA granules. Moreover, SDS, apart from its cell-disruptive function, also solubilizes hydrophobic components, which would otherwise contaminate PHA materials. The purity of obtained materials, as well as the yields of recovery, reach high values (values of purity higher than 99 wt.%, yields close to 1). Furthermore, we also focused on the removal of SDS from wastewater. The simple, inexpensive, and safe technique is based on the precipitation of SDS in the presence of KCl. The precipitate can be simply removed by decantation or centrifugation. Moreover, there is also the possibility to regenerate the SDS, which would substantially improve the economic feasibility of the process.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

NOVACKOVA, Ivana, Xenie KOURILOVA, Katerina MRAZOVA, Petr SEDLACEK, Michal KALINA, Vladislav KRZYZANEK, Martin KOLLER and Stanislav OBRUCA. Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles. Polymers. MDPI, 2022, vol. 14, No 9, p. 1761-1776. ISSN 2073-4360. Available from: https://dx.doi.org/10.3390/polym14091761.

@article{2262409,
   author = {Novackova, Ivana and Kourilova, Xenie and Mrazova, Katerina and Sedlacek, Petr and Kalina, Michal and Krzyzanek, Vladislav and Koller, Martin and Obruca, Stanislav},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/polym14091761},
   keywords = {polyhydroxyalkanoate (PHA); sodium dodecyl sulfate (SDS); PHA isolation; extremophiles; halophiles; thermophiles; Halomonas halophila; Schlegelella thermodepolymerans},
   language = {eng},
   issn = {2073-4360},
   journal = {Polymers},
   title = {Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles},
   url = {https://www.mdpi.com/2073-4360/14/9/1761},
   volume = {14},
   year = {2022}
}

TY  - JOUR
ID  - 2262409
AU  - Novackova, Ivana - Kourilova, Xenie - Mrazova, Katerina - Sedlacek, Petr - Kalina, Michal - Krzyzanek, Vladislav - Koller, Martin - Obruca, Stanislav
PY  - 2022
TI  - Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles
JF  - Polymers
VL  - 14
IS  - 9
SP  - 1761
EP  - 1761
PB  - MDPI
SN  - 20734360
KW  - polyhydroxyalkanoate (PHA)
KW  - sodium dodecyl sulfate (SDS)
KW  - PHA isolation
KW  - extremophiles
KW  - halophiles
KW  - thermophiles
KW  - Halomonas halophila
KW  - Schlegelella thermodepolymerans
UR  - https://www.mdpi.com/2073-4360/14/9/1761
N2  - Production of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage of the natural susceptibility of halophilic and thermophilic PHA producers to hypotonic lysis and we developed a simple and robust approach enabling effective isolation of PHA materials from microbial cells. The method is based on the exposition of microbial cells to hypotonic conditions induced by the diluted solution of sodium dodecyl sulfate (SDS) at elevated temperatures. Such conditions lead to disruption of the cells and release of PHA granules. Moreover, SDS, apart from its cell-disruptive function, also solubilizes hydrophobic components, which would otherwise contaminate PHA materials. The purity of obtained materials, as well as the yields of recovery, reach high values (values of purity higher than 99 wt.%, yields close to 1). Furthermore, we also focused on the removal of SDS from wastewater. The simple, inexpensive, and safe technique is based on the precipitation of SDS in the presence of KCl. The precipitate can be simply removed by decantation or centrifugation. Moreover, there is also the possibility to regenerate the SDS, which would substantially improve the economic feasibility of the process.
ER  -

NOVACKOVA, Ivana, Xenie KOURILOVA, Katerina MRAZOVA, Petr SEDLACEK, Michal KALINA, Vladislav KRZYZANEK, Martin KOLLER and Stanislav OBRUCA. Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles. \textit{Polymers}. MDPI, 2022, vol.~14, No~9, p.~1761-1776. ISSN~2073-4360. Available from: https://dx.doi.org/10.3390/polym14091761.

Detailed Information on Publication Record